Limited expression of a diverged beta-tubulin gene during soybean (Glycine max [L.] Merr.) development.
Limited expression of a diverged beta-tubulin gene during soybean (Glycine max [L.] Merr.) development.
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大豆 (Glycine max [L.] Merr.) 发育过程中分化的 β-微管蛋白基因的有限表达。
DOI：
10.1007/bf00020554
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发表时间：
1991
影响因子：
5.1
通讯作者：
Fosket,DE
中科院分区：
文献类型：
--
作者：
Han,IS;Jongewaard,I;Fosket,DE
我们研究了一个分化的大豆β-微管蛋白基因（命名为dsb-1）的发育表达，该基因已被克隆和测序。从3 ′端转录非翻译区序列构建了特异性的b-1基因探针。作为对照，从另一个大豆β-微管蛋白基因sb-2的第三外显子的高度保守区域构建了一个更通用的β-微管蛋白基因及其转录本的探针。从大豆不同组织器官中提取Poly（A）+RNA，用b-1基因特异性探针和β-微管蛋白通用探针交替检测。通用探针识别的β-微管蛋白转录物的水平在不同组织和器官中相差约3倍，并随器官发育状态而变化。最高水平的发现在年轻的，未展开的叶子，他们降低了叶片成熟发生。相比之下，转录sb-1几乎检测不到幼叶，他们增加叶片成熟发生。sb-1的转录水平在光生长植物的所有器官中都很低，除了下胚轴，在那里它们大约高出10倍。然而，最高水平的sb-1转录本观察到在伸长下胚轴的黄化幼苗。将6天龄的黄化幼苗暴露于光下12小时，下胚轴的进一步伸长停止，sb-1转录物的稳态水平急剧下降，接近100倍。
We examined the developmental expression of a diverged soybean β-tubulin gene (designatedsb-1), which had been cloned and sequenced previously. A probe specific for thesb-1gene was constructed from the 3′ transcribed untranslated sequence. As a control, a more general probe for β-tubulin genes and their transcripts was constructed from a highly conserved region of the third exon of another soybean β-tubulin gene,sb-2. Poly(A)+RNA, extracted from various soybean tissues and organs, was probed alternatively with thesb-1gene-specific probe and with the generic β-tubulin probe. Levels of β-tubulin transcripts recognized by the generic probe differed by a factor of approximately 3 in the different tissues and organs and varied with the state of organ development. Highest levels were found in young, unexpanded leaves and they decreased as leaf maturation occurred. In contrast, transcripts ofsb-1were nearly undetectable in young leaves, and they increased as leaf maturation occurred. Levels ofsb-1transcript were low in all organs of the light-grown plant examined, except the hypocotyl, where they were approximately 10-fold higher. However, the highest levels ofsb-1transcripts were observed in elongating hypocotyls of etiolated seedlings. Exposure of six-day-old etiolated seedlings to light for 12 hours halted further hypocotyl elongation and brought about a dramatic, nearly 100-fold, decrease in the steady-state level ofsb-1transcripts.