Characterization of vitellin, egg-specific protein and 30 kDa protein from Bombyx eggs, and their fates during oogenesis and embryogenesis
Characterization of vitellin, egg-specific protein and 30 kDa protein from Bombyx eggs, and their fates during oogenesis and embryogenesis
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DOI：
10.1016/0304-4165(86)90267-9
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发表时间：
1986-07
期刊：
影响因子：
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通讯作者：
Jiang Zhu;L. S. Indrasith;O. Yamashita
中科院分区：
文献类型：
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作者：
Jiang Zhu;L. S. Indrasith;O. Yamashita
用分子筛柱高效液相色谱法从家蚕卵提取液中分离纯化出卵黄蛋白、卵黄特异蛋白和30 kDa蛋白。用聚丙烯酰胺凝胶电泳法判断各制剂均为均一。卵黄蛋白是一个四聚体，分子质量为420 kDa，由两个重亚基(178 KDa)和两个轻亚基(43 KDa)组成；鸡蛋特异蛋白是由两个重亚基(72 KDa)和一个轻亚基(KDa)组成的三聚体(225 KDa)；30 kDa蛋白质是由三个单体(1，2和3)组成的混合物，分别由32.0，31.0和29.5 kDa的亚基组成。这三种蛋黄蛋白含有常见的氨基酸以及各种脂肪和碳水化合物。每种蛋白的抗血清均不发生交叉反应。卵黄蛋白、卵子特异蛋白和30 kDa蛋白在卵子发生过程中呈现不同的积累模式。在新产蛋中，卵黄蛋白、卵特异蛋白和30 kDa蛋白约占总蛋白的1/3。重量分别为40%、25%和35%。卵巢盘植入后雄虫发育的卵子缺乏卵黄蛋白，但卵特异性蛋白和30 kDa蛋白的含量与正常雌虫卵子相当。在胚胎发育过程中，卵子特异性蛋白得到了快速而充分的利用。大约35%的卵黄蛋白和50%的30 kDa蛋白未被使用，并被带到孵化的幼虫身上。卵黄蛋白的积累和利用与卵子发生和胚胎发生过程中卵黄蛋白的命运有关。森喜朗。
Three major yolk proteins, vitellin, egg-specific protein and 30 kDa proteins, were purified from the same extracts ofBombyx morieggs by high-performance liquid chromatography on a molecular sieving column. Each preparation was judged to be homogeneous by polyacrylamide gel electrophoresis. The subunit structure was estimated to be as follows: vitellin is a tetramer with a molecular mass of 420 kDa, consisting of two heavy subunits (178 kDa) and two light subunits (43 kDa); egg-specific protein is a trimer (225 kDa) of two heavy subunits (72 kDa) and one light subunit (64 kDa); 30 kDa proteins are a mixture of three monomers (1, 2 and 3) consisting of respective subunit molecular masses of 32.0, 31.0 and 29.5 kDa. The three yolk proteins contained the usual amino acids together with various lipids and carbohydrates. Antisera to each protein did not cross-react. The titration of vitellin, egg-specific protein and 30 kDa proteins on rocket immunoelectrophoresis showed a differential accumulation pattern during the course of oogenesis. In newly laid eggs, vitellin, egg-specific protein and 30 kDa proteins accounted for approx. 40%, 25% and 35%, respectively, in weight. The eggs developed in male hosts after implantation of ovary discs were deficient in vitellin but contained egg-specific protein and 30 kDa proteins at comparable levels to the normal female eggs. During embryogenesis, egg-specific protein was rapidly and completely utilized. Approx. 35% vitellin and 50% 30 kDa proteins remained unused and were carried over to the hatched larvae. Such accumulation and utilization of yolk proteins are correlated with the fates of the proteins during oogenesis and embryogenesis ofB. mori.