Tougu Xiaotong capsules may inhibit p38 MAPK pathway-mediated inflammation: In vivo and in vitro verification
Tougu Xiaotong capsules may inhibit p38 MAPK pathway-mediated inflammation: In vivo and in vitro verification
复制标题
透骨消痛胶囊可能抑制p38 MAPK通路介导的炎症：体内和体外验证
DOI：
10.1016/j.jep.2019.112390
复制
发表时间：
2020-03-01
影响因子：
5.4
通讯作者：
Asakawa, Tetsuya
中科院分区：
文献类型：
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作者：
Li, Xihai;Zhang, Zhenli;Asakawa, Tetsuya
民族药理学相关性：透骨消痛胶囊（TXC）是我国临床常用的治疗骨关节炎（OA）的中药复方，本研究的目的：通过体内外实验，探讨其对骨关节炎的治疗作用及其与p38丝裂原活化蛋白激酶（MAPK）通路的关系。通过观察改良Hulth模型（体内）和脂多糖（LPS）诱导的软骨退变和炎性因子来评价TXC的治疗作用。- 暴露的细胞模型（体外）。结果：TXC治疗逆转了动物和细胞OA模型中软骨退变相关的生物标志物（ADAMTS 4、ADAMTS 5、Col I、Col V、MMP 3、MMP 9和MMP 13）和炎症因子（IL-1 β、TNF-α和IL-6）。TXC给药后p-p38 MAPK表达下调，细胞模型中microRNA的变化恢复。结论：本研究证实了TXC在体内外对OA的治疗作用，提示TXC的治疗作用可能与p38 MAPK通路有关。
Ethnopharmacological relevance: Tougu Xiaotong capsules (TXC) are an herbal compound commonly used to treat osteoarthritis (OA) in China.Aim of the study: We attempted to verify TXC's therapeutic effects and mechanisms related to the p38 mitogen-activated protein kinase (MAPK) pathway in vivo and in vitro.Materials and methods: TXC's therapeutic effects were assessed by observing cartilage degeneration and inflammatory factors in a modified Hulth's model (in vivo) and a lipopolysaccharides (LPS)-exposed cellular model (in vitro). The expression of biomarkers related to p38 MAPK pathway-mediated inflammation was also investigated.Results: TXC treatment reversed cartilage degeneration related biomarkers (ADAMTS 4, ADAMTS 5, Col I, Col V, MMP 3, MMP 9, and MMP 13) and inflammation factors (IL-1 beta, TNF-alpha, and IL-6) in both the animal and cellular OA models. Expression of p-p38 MAPK was downregulated following TXC administration, and changes to microRNAs in the cellular models were recovered. These results indicated that the p38 MAPK pathway-related mechanism may involve therapeutic effects of TXC.Conclusions: This study verified TXC's efficacy to treat OA in vivo and in vitro and suggests that p38 MAPK pathway-related mechanisms may be involved in TXC's therapeutic effects.