Function of E-coli RNA polymerase sigma factor sigma(70) in promoter-proximal pausing
Function of E-coli RNA polymerase sigma factor sigma(70) in promoter-proximal pausing
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DOI：
10.1016/s0092-8674(00)80121-x
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发表时间：
1996-08-09
期刊：
影响因子：
64.5
通讯作者：
Yarnell, WS
中科院分区：
文献类型：
--
作者：
Ring, BZ;Yarnell, WS
E.大肠杆菌RNA聚合酶不仅在起始阶段起作用，而且在延伸的早期阶段起作用，以诱导转录暂停，并同时允许噬菌体λ基因Q转录抗终止子起作用。我们鉴定了DNA中诱导早期暂停的信号是sigma（70）-10启动子共有序列的一个版本，并且我们表明sigma（70）对于暂停是必需的，并且存在于暂停的转录复合物中。sigma（70）的区域2和3足以引起暂停。由于暂停是由开放转录泡的非模板DNA链诱导的，我们得出结论，含有sigma（70）的RNA聚合酶将非模板链作为单链DNA进行碱基特异性识别。我们认为，当-10启动子接触被破坏时，sigma（70）仍然与核心RNA聚合酶结合，然后移动到暂停诱导序列。
The sigma factor sigma(70) of E. coli RNA polymerase acts not only in initiation, but also at an early stage of elongation to induce a transcription pause, and simultaneously to allow the phage lambda gene Q transcription antiterminator to act. We identify the signal in DNA that induces early pausing to be a version of the sigma(70) -10 promoter consensus, and we show that sigma(70) is both necessary for pausing and present in the paused transcription complex. Regions 2 and 3 of sigma(70) suffice to induce pausing. Since pausing is induced by the nontemplate DNA strand of the open transcription bubble, we conclude that RNA polymerase containing sigma(70) carries out base-specific recognition of the nontemplate strand as single stranded DNA. We suggest that sigma(70) remains bound to core RNA polymerase when the -10 promoter contacts are broken, and then moves to the pause-inducing sequence.