Photoinitiating polymerization to prepare Biocompatible chitosan hydrogels
Photoinitiating polymerization to prepare Biocompatible chitosan hydrogels
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DOI：
10.1002/app.28704
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发表时间：
2008-10-15
影响因子：
3
通讯作者：
Gao, Changyou
中科院分区：
文献类型：
--
作者：
Hu, Xiaohong;Gao, Changyou
以水溶性壳聚糖衍生物（chitosan‐MA‐LA，CML）为原料，在Irgacure 2959存在下，在紫外光照射下，通过光引发聚合制备了壳聚糖水凝胶。通过壳聚糖的胺基与乳酸和甲基丙烯酸的酰胺化得到CML。水凝胶的凝胶化时间可以在5-50 min范围内调节，并且可以通过诸如MA取代度、引发剂浓度、氧和盐的存在等因素来控制。干燥的水凝胶吸附数十至数百倍的水，形成高度水合的凝胶。MA取代度越高、pH值越高、盐浓度越高，溶胀率越小。流变学测试表明，该水凝胶在测量频率范围内具有弹性，储能模量和损耗模量分别为0.8-7 kPa和10-100 Pa，软骨细胞体外培养结果表明，软骨细胞在该水凝胶的提取液中可以正常增殖，且在较低引发剂浓度下无细胞毒性。相比之下，由氧化还原引发体系制备的水凝胶的萃取剂，即，过硫酸铵（APS）和N，N，N′，N′-四甲基乙二胺（TEMED）具有明显的细胞毒性。因此，Irgacure 2959引发的壳聚糖水凝胶具有更好的综合性能，特别是生物相容性更好，更适合于生物医学应用。© 2008 Wiley Periodicals，Inc.应用聚合物科学杂志，2008年
Chitosan hydrogels were prepared from water soluble chitosan derivatives (chitosan‐MA‐LA, CML) by photoinitiating polymerization under the existence of Irgacure2959 and the irradiation of UV light. The CML was obtained by amidation of the amine groups of chitosan with lactic acid and methacrylic acid. Gelation time of the hydrogel could be adjusted within a range of 5–50 min, and controlled by factors such as the degree of MA substitution, initiator concentration, existence of oxygen, and salt. The dry hydrogel adsorbed tens to hundred times of water, forming a highly hydrated gel. The swelling ratio was smaller at the higher degree of MA substitution, higher pH, and higher salt concentration. Rheological test showed that the hydrogel is elastomeric in the measuring frequency range, with a storage modulus and loss modulus of 0.8–7 kPa and 10–100 Pa, respectively.In vitroculture of chondrocytes demonstrated that the cells could normally proliferate in the extractant of the hydrogels, showing no cytotoxicity at lower initiator concentration. By contrast, the extractant of the hydrogel made by the redox initiating system, i.e., ammonium persulfate (APS) andN,N,N′,N′‐tetramethylethylenediamine (TEMED), showed apparent cytotoxicity. Thus, the chitosan hydrogels initiated by the Irgacure2959 have better comprehensive properties, in particular better biocompatibility, and are more suitable for biomedical applications. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008