Comparative enzymatic study of HPLC-fractionated Crotalus venoms.
Comparative enzymatic study of HPLC-fractionated Crotalus venoms.
复制标题
HPLC 分级分离响尾蛇毒液的比较酶学研究。
DOI：
10.1016/0305-0491(89)90056-4
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Minton,SA
中科院分区：
文献类型：
--
作者：
Soto,JG;Perez,JC;Lopez,MM;Martinez,M;Quintanilla-Hernandez,TB;Santa-Hernandez,MS;Turner,K;Glenn,JL;Straight,RC;Minton,SA
1.用阴离子交换层析和阳离子交换层析的方法，对鳄鱼属的10种毒液(大口鱼、大口鱼、硬枝巴鱼、可怕大巴鱼、大斑大鱼、多头大嘴巴鱼、大口大嘴巴鱼、软体大嘴巴鱼、大口巴豆象、大口巴豆象、黄斑大口鱼)进行了分离。2.对高效液相色谱组分的出血性、溶血性和蛋白水解性进行了检测。3.弗吉尼亚负鼠(Didelphis Viginiana)粗品血清中和了高效液相色谱组分的出血活性。
1. Ten venoms of the genus Crotalus (Crotalus adamanteus, Crotalus atrox, Crotalus durissus durissus, Crotalus horridus horridus, Crotalus lepidus, Crotalus polystictus, Crotalus molossus molossus, Crotalus pusillus, Crotalus scutulatus scutulatus, venom B, and Crotalus viridis lutosus) were fractionated using HPLC anion and cation exchange chromatography. 2. HPLC venom fractions were tested for hemorrhagic, hemolytic, and proteolytic activities. 3. Crude Virginia opossum (Didelphis virginiana) serum neutralized the hemorrhagic activity of HPLC fractions.