Bovine Cryptosporidium parvum field isolates differ in cytopathogenicity in HCT-8 monolayers
Bovine Cryptosporidium parvum field isolates differ in cytopathogenicity in HCT-8 monolayers
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DOI：
10.1016/j.vetpar.2019.08.006
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发表时间：
2019-09-01
影响因子：
2.6
通讯作者：
Daugschies, A.
中科院分区：
文献类型：
--
作者：
Holzhausen, I;Lendner, M.;Daugschies, A.
哺乳的小牛容易感染隐孢子虫。寄主的保存条件和免疫状态影响着临床病情的变化程度，但菌株毒力的多样性也可能起到一定作用。本研究的目的是通过Hct-8细胞单层的MU试验评价26株微小隐孢子虫野外分离株的细胞病变效应。接种野生菌卵囊的单层细胞存活率差异较大，分别为17.7%(+/-5.1%)和99.5%(+/-7.1%)。室内参考菌株的细胞活力的标准偏差为18.6%，在每次检测中都进行了联合测试。将田间分离株分成三类细胞致病性。可能储存时间的长短对体外感染后检测到的细胞破坏类别的水平有影响。该应用工具可能有助于更好地了解隐孢子虫病在现场的变化过程。
Suckling calves are prone to Cryptosporidium infection. The variable degree of clinical disease is influenced by keeping conditions and immune status of the host, but diversity of isolate virulence may also contribute. The aim of the current study was to evaluate the cytopathogenic effects of 26 C. parvum field isolates by using a MU assay in HCT-8 cell monolayers. Cell viability of monolayers inoculated with oocysts of the field isolates varied considerably with values of 17.7% (+/- 5.1%) to 99.5% (+/- 7.1%). A standard deviation of 18.6% was detected for cell viability of the in house reference strain, which were tested alongside in every assay. Field isolates were grouped in three categories of cytopathogenicity. Probably the length of storage has an effect on the level of the cell destruction category detected post infection in vitro. The applied tool may help to better understand the variable course of cryptosporidiosis in the field.