The effect of trimethylamine N‐oxide on Helicobacter pylori‐induced changes of immunoinflammatory genes expression in gastric epithelial cells
The effect of trimethylamine N‐oxide on Helicobacter pylori‐induced changes of immunoinflammatory genes expression in gastric epithelial cells
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DOI：
10.1016/j.intimp.2016.11.032
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发表时间：
2017-02
影响因子：
5.6
通讯作者：
Daoyan Wu;M. Cao;Jingshan Peng;Ningzhe Li;Sijun Yi;Liju Song;Xuege Wang;Mao Zhang;Jian Zhao
中科院分区：
文献类型：
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作者：
Daoyan Wu;M. Cao;Jingshan Peng;Ningzhe Li;Sijun Yi;Liju Song;Xuege Wang;Mao Zhang;Jian Zhao
幽门螺杆菌（H. pylori）诱导胃粘膜的免疫和炎症反应。氧化三甲胺（TMAO），来自饮食和代谢产物，通过肠道微生物群的作用，与炎症性疾病有关。探讨TMAO和H.本研究应用人类基因芯片AffyesthetHTA 2.0检测幽门螺杆菌感染对胃上皮细胞基因表达的影响。结果表明，H. pylori和TMAO联合治疗与对照相比。GO和KEGG分析表明，这些差异表达基因的功能与免疫炎症密切相关。GO网络分析表明Toll样受体信号通路是最重要的生物学过程，共获得49个免疫炎症相关上调基因。H. pylori和TMAO感染后，IL-6、CXCL 1、CXCL 2、FOS和C3等免疫炎症相关基因的表达均明显增强。幽门感染的细胞和TMAO刺激的细胞。RT-PCR检测IL-6、CXCL 1的表达水平。此外，与H.幽门感染细胞本研究提示TMAO改变了H诱导的免疫炎症基因的表达水平。pylori感染，以及H. pylori和TMAO的研究为慢性胃炎、胃溃疡和胃癌的发生发展提供了新的见解。
Colonization of Helicobacter pylori (H. pylori) induces immune and inflammatory response in gastric mucosa. TrimethylamineN-oxide (TMAO), from diet and metabolite through the action of gut microbiota, has been linked to inflammatory diseases. To investigate the effects of TMAO andH. pyloriinfection on gene expression in gastric epithelial cells, Human gene chip Affymetrix HTA 2.0 was used in this study. 1312 genes were identified as differentially expressed genes in GES-1 cells withH. pyloriand TMAO co-treatment compared to the control. GO and KEGG analyses indicated that the functions of these differentially expressed genes were related closely with immune inflammation. GO-network showed that Toll-like receptor signaling pathway was the most important biological processes and 49 up-regulated genes related to immune inflammation were obtained. The synergistic effects ofH. pyloriand TMAO enhanced the genes expression of IL-6, CXCL1, CXCL2, FOS and C3 related to immune inflammation in comparison with those of non-infected control cells,H. pylori-infected cells, and TMAO-stimulated cells. RT-PCR verified the expression levels of IL-6, CXCL1. Additionally, expression levels of 2053 genes were altered and 52 immunoinflammatory genes were upregulated in comparison withH. pylori-infected cells. This study suggested that TMAO altered the expression levels of immunoinflammatory genes induced byH. pyloriinfection, and the synergistic effects ofH. pyloriand TMAO provided novel insights into the development of chronic gastritis, gastric ulcer and gastric cancer.