Protective effect of galangin in Concanavalin A-induced hepatitis in mice
Protective effect of galangin in Concanavalin A-induced hepatitis in mice
复制标题
高良姜素对伴刀豆球蛋白A诱导的小鼠肝炎的保护作用
DOI：
10.2147/dddt.s80979
复制
发表时间：
2015-01-01
影响因子：
4.8
通讯作者：
Chen, Fuxiang
中科院分区：
文献类型：
--
作者：
Luo, Qingqiong;Zhu, Liping;Chen, Fuxiang
高良姜素是从高良姜（Alpinia officinarum Hance）和高良姜（Alpinia officinarum Hance）中提取的一种活性药理成分，据报道具有抗炎和抗氧化特性。本研究旨在揭示高良姜对伴刀豆球蛋白A（ConA）诱导的肝炎（CIH）（一种成熟的免疫介导的肝损伤动物模型）的影响，并阐明相关机制。用高良姜素预处理C57 BL/6小鼠，然后用ConA攻击。结果表明，高良姜素抑制ConA诱导的肝损伤。与用溶剂溶液预处理的对照小鼠相比，用高良姜素预处理的小鼠显示出更多的肝损伤减轻。在高良姜素预处理的CIH小鼠中，几种炎性细胞因子（包括肿瘤坏死因子-α、干扰素-γ和白细胞介素-12）血清水平的升高显著减弱，肝脏中趋化因子和粘附分子（如干扰素诱导蛋白-10、巨噬细胞炎性蛋白-1 α和细胞间粘附分子-1信使RNA）表达降低。此外，用高良姜素预处理的CIH小鼠在肝脏中表现出较少的白细胞浸润和T细胞活化。此外，高良姜素的抗炎作用的机制可能归因于其对关键炎症信号传导途径的调节，包括核因子κ B和干扰素-γ/信号转导子和转录激活子1。总的来说，这些发现表明高良姜素在体内免疫介导的肝损伤中的预防和治疗潜力。
Galangin is an active pharmacological ingredient from propolis and Alpinia officinarum Hance, and has been reported to have anti-inflammatory and antioxidative properties. The present study aims to reveal the effect of galangin on Concanavalin A (ConA)-induced hepatitis (CIH), a well-established animal model of immune-mediated liver injury, and to clarify the related mechanism. C57BL/6 mice were pretreated with galangin followed by ConA challenge. Results indicated that galangin inhibited ConA-induced liver damage. Mice pretreated with galangin showed more reduction of liver damage when compared with control mice pretreated with vehicle solution. In galangin-pretreated mice with induced CIH, increases in serum levels of several inflammatory cytokines, including tumor necrosis factor-α, interferon-γ, and interleukin-12 were dramatically attenuated, and chemokines and adhesion molecules like interferon inducible protein-10, macrophage inflammatory protein-1α, and inter-cellular adhesion molecule-1 messenger RNA expressions in liver were decreased. Moreover, CIH mice pretreated with galangin showed less leukocyte infiltration and T-cell activation in the liver. Further, the mechanism of the anti-inflammatory effects of galangin may be attributed to its modulation of crucial inflammatory signaling pathways, including nuclear factor kappa B and interferon-gamma/signal transducer and activator of transcription 1. Collectively, these findings suggest the preventive and therapeutic potential of galangin in immune-mediated liver injury in vivo.