A highly efficient method of inducing sex change using social control in the protogynous orange-spotted grouper (Epinephelus coioides)
A highly efficient method of inducing sex change using social control in the protogynous orange-spotted grouper (Epinephelus coioides)
复制标题
一种利用社会控制诱导雌性橙斑石斑鱼（Epinephelus coioides）性别改变的高效方法
DOI：
10.1016/j.aquaculture.2019.734787
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发表时间：
2020-02-25
期刊：
影响因子：
4.5
通讯作者：
Zhang, Y.
中科院分区：
文献类型：
--
作者：
Chen, J.;Chen, H.;Zhang, Y.
在这项研究中，开发了一种高效的方法，诱导性别转换的原始雌核发育的橙点石斑鱼（Epinealphanus coioides）的社会控制。通过一系列实验，研究了社会单位大小和季节性对橙点石斑鱼性别转换率的影响。结果表明，在繁殖前和繁殖季节，在有三个雌性的社会单位中去除雄鱼是有史以来最快的诱导橙斑石斑鱼人工变性的方法。数据表明，性别变化通常发生在社会单位中最大的女性身上。血清中雄激素和皮质醇水平的急剧增加被认为是女性向男性性别转变的标志。使用本研究中描述的方法，从女性到男性的性别变化发生在将男性从社会单位中移除的两到四周内。为了检查精子生育力，分析了通过诱导变性获得的雄性的受精率。结果表明，通过这种方法获得的雄性可以成功地使成熟卵受精。该方法可解决人工养殖条件下石斑鱼产卵所需雄性个体数量不足的问题，促进石斑鱼养殖业的发展。
In this study, a highly efficient method of inducing sex change using social control in the protogynous orange-spotted grouper (Epinephelus coioides) was developed. A series of experiments were carried out to investigate the influence of social unit size and seasonality on the rate of sex change rate in the orange-spotted grouper. The results revealed that the removal of male fish in social units with three females during the pre-breeding and breeding seasons was the fastest method ever recorded for inducing an artificial sex change in orange-spotted grouper. The data indicated that a sex change generally occurred in the largest female in the social unit. A sharp increase in levels of androgen and cortisol in serum are proposed as a marker of the onset of a female-to-male sex change. Using the method described in this study, a sex change from female to male occurred within two to four weeks of removing males from the social unit. To examine sperm fertility, the fertilization rate of males obtained by induced sex change was analyzed. The results revealed that males obtained by this method can successfully fertilize mature eggs. This method could address the scarcity of male groupers required for spawning in captivity, and would promote the development of grouper aquaculture.