ISOLATION AND CHARACTERIZATION OF RETICULOENDOTHELIOSIS VIRUS TRANSFORMED BONE-MARROW CELLS
ISOLATION AND CHARACTERIZATION OF RETICULOENDOTHELIOSIS VIRUS TRANSFORMED BONE-MARROW CELLS
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DOI：
10.1159/000149771
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发表时间：
1974-01-01
期刊：
影响因子：
4.6
通讯作者：
BOSE, HR
中科院分区：
文献类型：
--
作者：
FRANKLIN, RB;MALDONADO, RL;BOSE, HR
从感染网状内皮组织增生症病毒(REV)的垂死雏鸡的骨髓中分离出转化细胞。这些细胞已经在体外连续传代了30多代。当这些细胞接种到1日龄鸡的翼网中时，会诱发实体瘤，而无细胞滤液则会诱导网状内皮组织增生症。在蔗糖密度为1.16g/cm3的条件下，骨髓细胞与鸡胚胎细胞带共培养后病毒颗粒被回收。病毒粒子含有64S和4S RNA种类，其多肽组成与REV相似，与Rous肉瘤病毒不同。补体结合试验表明，从这些培养物中浓缩的病毒中不存在禽白血病-肉瘤复合体病毒。
Transformed cells have been isolated from the bone marrow of reticuloendotheliosis virus (REV)-infected, moribund chicks. These cells have been maintained in vitro through more than 30 serial passages. The cells induce solid tumors when inoculated into the wing web of day-old chickens, and cell-free filtrates induce reticuloendotheliosis. Virus particles recovered after cocultivation of bone marrow cells and chicken embryo cells band at a density of 1.16 g/cm3in sucrose gradients. The virions contain 64S and 4S RNA species and a polypeptide composition similar to that of REV and distinct from that of Rous sarcoma virus. Complement fixation tests indicate that viruses of the avian leukosis-sarcoma complex are not present in virus concentrated from these cultures.