Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: A review
Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: A review
复制标题
DOI：
10.1109/tps.2006.878381
复制
发表时间：
2006-08-01
影响因子：
1.5
通讯作者：
Georghiou, George E.
中科院分区：
文献类型：
--
作者：
Gaunt, Lindsey F.;Beggs, Clive B.;Georghiou, George E.
在空气中使用常压非热气体放电进行生物去污是目前重大研究工作的主题。细菌失活的机制有很多猜测，特别是关于离子和活性气体物种的作用。已经提出了两种机制：静电破坏细胞膜和致命性氧化细胞膜或细胞质成分。结果表明，在革兰氏阴性菌中，死亡伴随着细胞的裂解和碎裂，而在革兰氏阳性菌中，尽管通常观察到细胞质渗漏，但死亡并不伴随。气体放电可能是带电粒子、离子、活性气体物种、自由基和辐射(紫外线、红外和可见光)的来源，其中许多都具有有案可查的生物杀灭特性。这些机构在去污过程中发挥的个别作用还没有得到很好的理解或量化。然而，一些物种与生物分子的反应在文献中另有记载。对氧化应激的研究相对较多，暴露在空气中的气体排放很可能会引起极端的氧化挑战。本文综述了常压低温等离子体产生的主要反应物种以及它们与生物分子的已知反应。随着基于等离子体的净化和杀菌设备在医疗、保健、食品加工和空气净化应用中的广泛应用，了解这些机制变得越来越重要。提出了一些方法来阐明活性物种的相对重要性。
Biological decontamination using a nonthermal gas discharge at atmospheric pressure in air is the subject of significant research effort at this time. The mechanism for bacterial deactivation undergoes a lot of speculation, particularly with regard to the role of ions and reactive gas species. Two mechanisms have been proposed: electrostatic disruption of cell membranes and lethal oxidation of membrane or cytoplasmic components. Results show that death is accompanied by cell lysis and fragmentation in Gram-negative bacteria but not Gram-positive species, although cytoplasmic leakage is generally observed. Gas discharges can be a source of charged particles, ions, reactive gas species, radicals, and radiation (ultraviolet, infrared, and visible), many of which have documented biocidal properties. The individual roles played by these in decontamination are not well understood or quantified. However, the reactions of some species with bioniolecules are documented otherwise in the literature. Oxidative stress is relatively well studied, and it is likely that exposure to gas discharges in air causes extreme oxidative challenge. In this paper, a review is presented of the major reactive species generated by nonthermal plasma at atmospheric pressure and the known reactions of these with biological molecules. Understanding these mechanisms becomes increasingly important as plasma-based decontamination and sterilization devices come closer to a wide-scale application in medical, healthcare, food processing, and air purification applications. Approaches are proposed to elucidate the relative importance of reactive species.