Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene.
Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene.
复制标题
单纯疱疹病毒2型胸苷激酶基因的核苷酸序列。
DOI：
10.1128/jvi.46.3.1045-1050.1983
复制
发表时间：
1983
影响因子：
5.4
通讯作者：
Galloway,DA
中科院分区：
文献类型：
--
作者：
Swain,MA;Galloway,DA
我们已经确定了完整的核苷酸序列的单纯疱疹病毒（HSV）2型株333胸苷激酶基因。胸苷激酶基因的序列显示出1，128个核苷酸的开放翻译阅读框，编码376个氨基酸的蛋白质。将该基因序列与MP株HSV 1型胸苷激酶基因序列进行比较。L. McKnight，Nucleic Acids Res. 8：5949 - 5964，1980）和来自菌株CL 101（M.瓦格纳，J.A. Sharp和W. C. Summers，Proc. Natl. Acad. sci. U.S.A. 78：1441 - 1445，1981）来评估一个病毒基因的型内和型间变异的程度。编码结构基因的核苷酸在两种HSV 1型毒株之间的差异为1.7%，在HSV 1型和HSV 2型之间的差异为19%，这两种蛋白质的氨基酸序列的差异分别为1.9%和27%。编码5 '端调控序列的DNA比编码结构基因的DNA保守，3'端的DNA同源性最低。
We have determined the complete nucleotide sequence of the thymidine kinase gene of herpes simplex virus (HSV) type 2 strain 333. The sequence of the thymidine kinase gene exhibits an open translational reading frame of 1,128 nucleotides encoding a protein of 376 amino acids. The DNA sequence was compared with that of the HSV type 1 thymidine kinase gene from strain MP (S. L. McKnight, Nucleic Acids Res. 8:5949-5964, 1980) and from strain CL 101 (M. J. Wagner, J. A. Sharp, and W. C. Summers, Proc. Natl. Acad. Sci. U.S.A. 78:1441-1445, 1981) to assess the extent of intra- and intertypic variation for one viral gene. The nucleotides encoding the structural gene varied 1.7% between the two HSV type 1 strains and 19% between HSV type 1 and HSV type 2, which translated to differences in the amino acid sequence of the two proteins of 1.9 and 27%, respectively. The DNA encoding the 5' regulatory sequences appeared to be more conserved than the DNA coding for the structural gene, and the DNA at the 3' end of the gene was the least homologous.