Overexpression of centrosomal protein 55 regulates the proliferation of glioma cell and mediates proliferation promoted by EGFRvIII in glioblastoma U251 cells
Overexpression of centrosomal protein 55 regulates the proliferation of glioma cell and mediates proliferation promoted by EGFRvIII in glioblastoma U251 cells
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中心体蛋白55的过表达调节胶质瘤细胞的增殖并介导胶质母细胞瘤U251细胞中EGFRvIII促进的增殖
DOI：
10.3892/ol.2017.7573
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发表时间：
2017
期刊：
影响因子：
2.9
通讯作者：
GUANGHUI LI
中科院分区：
文献类型：
--
作者：
HONGFAN ZHU;DIANGANG CHEN;JINLIANG TANG;CHANGLIN HUANG;SHENGQING LV;DONGLIN WANG;GUANGHUI LI
表皮生长因子受体（EGFR）在胶质瘤中经常被扩增，最常见的细胞外结构域突变是EGFR变体III （EGFRvIII）。异常EGFRvIII信号传导已被证明在驱动肿瘤进展中起重要作用。中心体蛋白55 （CEP55）是中心体相关蛋白家族的一员，参与细胞周期的细胞质分裂。它存在于少数正常组织和各种肿瘤细胞中。CEP55在胶质瘤细胞中的表达和功能有待进一步研究。本研究在40例胶质瘤组织和10例非肿瘤脑组织中检测到CEP55的表达。转染EGFRvIII和CEP55 siRNA后，分析胶质母细胞瘤U251细胞的增殖情况。我们发现CEP55在胶质瘤组织中的表达明显高于正常脑组织。转染EGFRvIII后，U251细胞的增殖明显增强。CEP55的敲低抑制了U251细胞的增殖，能够消除EGFRvIII对U251细胞的促增殖作用。CEP55在胶质瘤细胞的增殖和介导EGFRvIII刺激的胶质瘤细胞增殖中发挥关键作用。CEP55可能是表达EGFRvIII的胶质瘤患者的一个新的分子治疗靶点。
The epidermal growth factor receptor (EGFR) is often amplified in glioma, with the most common extracellular domain mutation being EGFR variant III (EGFRvIII). Abnormal EGFRvIII signaling has been shown to be important in driving tumor progression. Centrosomal protein 55 (CEP55), a member of the centrosomal relative proteins family, participates cytokinesis in the cell cycle. It exists in a few normal tissues and various tumor cells. The expression and function of CEP55 in human glioma cells need to investigate. In this study, the expression of CEP55 was detected in 40 cases of glioma tissues and 10 cases of non‑tumor brain tissue. The proliferation of glioblastoma U251 cells was analyzed after transfection with EGFRvIII and CEP55 siRNA. We found that the expression of CEP55 was increased significantly in the glioma tissues than in normal brain tissue. The proliferation of U251 cells increased remarkably after transfection with EGFRvIII. Knockdown of CEP55 inhibited proliferation of U251 cells and was able to eliminate the effect of promoting proliferation induced by EGFRvIII in U251 cells. CEP55 played a key role in the proliferation of glioma cells and mediated EGFRvIII‑stimulated proliferation in glioma cells. CEP55 might be a novel molecular therapeutic target in patients with gliomas expressing EGFRvIII.