Macrophages within NSCLC tumour islets are predominantly of a cytotoxic M1 phenotype associated with extended survival
Macrophages within NSCLC tumour islets are predominantly of a cytotoxic M1 phenotype associated with extended survival
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DOI：
10.1183/09031936.00065708
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发表时间：
2009-01-01
影响因子：
24.3
通讯作者：
Bradding, P.
中科院分区：
文献类型：
--
作者：
Ohri, C. M.;Shikotra, A.;Bradding, P.
在肿瘤胰岛中表达大量巨噬细胞的非小细胞肺癌（NSCLC）患者有明显的生存优势。本研究的主要目的是确定非小细胞肺癌相关巨噬细胞的免疫表型。应用免疫组化技术，在20例生存期延长（中位92.7个月）和20例生存期较差（中位7.7个月）的患者手术切除肿瘤的胰岛和基质中鉴定了表达细胞毒性M1表型或非细胞毒性M2表型标记物的CD68（+）巨噬细胞。与生存期较差的患者相比，延长患者的胰岛M1和M2巨噬细胞密度均明显增加。延长生存组M1型胰岛细胞密度较M2型明显增加，M1标记物阳性的胰岛巨噬细胞占70%，M2标记物阳性的胰岛巨噬细胞占38%，M1型巨噬细胞的胰岛：基质比例较M2型明显增加。胰岛M1巨噬细胞高于和低于中位数表达的患者的5年生存率分别为75岁和75岁
There is a marked survival advantage for patients with nonsmall cell lung cancer (NSCLC) expressing high numbers of macrophages in their tumour islets. The primary aim of the present study was to determine the immunological phenotype of NSCLC-associated macrophages.CD68(+) macrophages expressing markers of a cytotoxic M1 phenotype or a noncytotoxic M2 phenotype were identified in the islets and stroma of surgically resected tumours from 20 patients with extended survival (median 92.7 months) and 20 with poor survival (median 7.7 months), using immunohistochemistry.The islet density of both M1 and M2 macrophages was markedly increased in extended compared with poor survival patients. In the extended survival group, M1 islet density was significantly increased compared with M2 density, 70% of islet macrophages were positive for M1 markers versus 38% for M2, and the islet:stromal ratio of M1 macrophages was markedly increased compared with M2. The 5-yr survival for patients with above and below median expression of M1 macrophages in the islets was >75 and