Long-term chronological shifts in bacterial communities and hydrolytic extracellular enzyme activities in the forty years following a land-use change from upland fields to paddy fields
Long-term chronological shifts in bacterial communities and hydrolytic extracellular enzyme activities in the forty years following a land-use change from upland fields to paddy fields
复制标题
土地利用从旱地变为水田后四十年中细菌群落和水解胞外酶活性的长期时间变化
DOI：
10.1016/j.pedobi.2015.12.002
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发表时间：
2016-01-01
期刊：
影响因子：
2.3
通讯作者：
Chen, Lijun
中科院分区：
文献类型：
--
作者：
Jiang, Nan;Wei, Kai;Chen, Lijun
在这里，我们使用了40年的时间序列的土地利用变化，从高地到水田，研究短期和长期的时间模式的细菌群落和胞外酶活性（EEAs）。在不同的时间点（研究前1，5，10，20，30和40年），从一高地和6个水田，建立在前高地在两个深度（0-20厘米和20-40厘米）的土壤样品。454条形码焦磷酸测序用于鉴定每个土壤样品中存在的细菌。土地利用变化后，细菌多样性减少，细菌群落组成在短期内发生变化;然而，社区表现出一定程度的弹性，在结构和组成上恢复到与土地利用变化前的原始状态相似的状态。此外，不同的细菌反应观察到的两个土壤深度。土壤化学性质与其中一些变化有关;全磷（TP）、土壤pH和奥尔森磷（OP）与细菌群落显着相关，而土壤pH和养分含量均显着影响EEA。此外，我们发现的证据表明EEAs和特定细菌属的丰度之间的关联，特别是绿杆菌和变形菌。总的来说，这项研究提供了一个时间视图的细菌群落组装和功能转变后，土地利用变化。(C)2015 Elsevier GmbH. All rights reserved.
Here, we used a forty-year chronosequence of land-use change from upland fields to paddy fields to study short- and long-term temporal patterns in bacterial communities and extracellular enzyme activities (EEAs). Soil samples were collected at two depths (0-20 cm and 20-40 cm) from one upland field and six paddy fields that were established on former upland fields at different time points (1, 5, 10, 20, 30, and 40 years before the study). 454 barcoded-pyrosequencing was used to identify the bacteria residing in each soil sample. Following the land-use change, bacterial diversity was reduced and bacterial community composition changed in the short-term; however, communities demonstrated a certain level of resilience, returning in structure and composition to states that resembled their original states, prior to land-use change, during the first two decades. In addition, different bacterial responses were observed for the two soil depths. Soil chemical properties were associated with some of these changes; total phosphorus (TP), soil pH and Olsen phosphorus (OP) were significantly correlated with bacterial communities, while both soil pH and nutrient contents significantly affected the EEAs. In addition, we found evidence suggesting associations between EEAs and the abundance of specific bacterial genera, particularly to Chloroflexi and Proteobacteria. Overall, this study provides a temporal view of the bacterial community assembly and functional shifts following land-use change. (C) 2015 Elsevier GmbH. All rights reserved.