Chemical identification of nestin-immunoreactive neurons in the rat basal forebrain: A re-examination
Chemical identification of nestin-immunoreactive neurons in the rat basal forebrain: A re-examination
复制标题
大鼠基底前脑中巢蛋白免疫反应性神经元的化学鉴定：重新检查
DOI：
10.1016/j.neuint.2010.02.005
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发表时间：
2010-04-01
影响因子：
4.2
通讯作者：
Li, Dong-Pei
中科院分区：
文献类型：
--
作者：
Guo, Kai-Hua;Zhu, Jian-Hua;Li, Dong-Pei
本研究探讨了最近发现的神经元表达蛋白巢蛋白在内侧隔对角带的布罗卡（MS-DBB）的成年大鼠和人类。已知来自MS-DUB的这些巢蛋白阳性神经元投射到大脑的海马和额叶皮质。然而，它们的化学鉴定尚未完全阐明。在这项研究中，我们进一步研究了化学身份的nestin免疫反应（ir）神经元在大鼠使用双重免疫荧光标记和单细胞逆转录聚合酶链反应（RT-PCR）技术。双标记结果显示，基底前脑MS DBB区所有Nestin阳性神经元均呈胆碱乙酰转移酶（ChAT）免疫反应。相反，只有约43%的ChAT免疫阳性神经元显示nestin免疫反应。此外，绝大多数nestin免疫阳性神经元（95%）是神经生长因子受体（NGFR）阳性。Nestin阳性神经元主要分布于MS-DBB的吻侧区和中间区。单细胞RT-PCR结果显示，90%表达Nestin mRNA的神经元也表达ChAT mRNA，但谷氨酸脱羧酶67（GAD 67）和囊泡谷氨酸转运体（VGLUT）的mRNA均不表达。这些结果提供了第一个证据，nestin免疫反应神经元在基底前脑是一个亚群的经典胆碱能神经元。在nestin免疫阳性神经元中NGFR蛋白的高活化，我们认为nestin在胆碱能神经元中的存在可能与胆碱能神经元的存活和可塑性有关。(C)2010爱思唯尔有限公司版权所有。
This study explores recently identified neurons that express the protein nestin in the medial septum-diagonal band of Broca (MS-DBB) of adult rats and humans. These nestin positive neurons from MS-DUB are known to project to the hippocampus and frontal cortex of the brain. However, their chemical identification has not been fully elucidated. In this study, we further investigated the chemical identity of the nestin-immunoreactive (ir) neurons in rats using double immunofluorescence labeling and single cell reverse transcription polymerase chain reaction (RT-PCR) techniques. The results of double labeling showed that all nestin-ir neurons exhibited choline acetyltransferase (ChAT) immunoreactivity in the MS-DBB of the basal forebrain. Conversely, only about 43% of the ChAT-ir neurons showed nestin immunoreactivity. In addition, a vast majority of the nestin-ir neurons (95%) were nerve growth factor receptor (NGFR) positive. The nestin-ir neurons were highly distributed in the rostral and intermediated regions of the MS-DBB. Single cell RT-PCR results showed that 90% of the nestin mRNA expressed neurons displayed ChAT mRNA expression as well, but neither the mRNA of the proteins glutamic acid decarboxylases 67 (GAD67) nor vesicular glutamate transporters (VGLUTs). These results provide the first evidence that nestin-ir neurons in the basal forebrain are a subpopulation of the classic cholinergic neurons. With high NGFR proteins activated in the nestin-ir neurons, we propose that the presence of nestin in the cholinergic neurons might be related to the survival and plasticity of the cholinergic neurons. (C) 2010 Elsevier Ltd. All rights reserved.