The formation of the rodlet layer of streptomycetes is the result of the interplay between rodlins and chaplins
The formation of the rodlet layer of streptomycetes is the result of the interplay between rodlins and chaplins
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DOI：
10.1111/j.1365-2958.2004.04143.x
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发表时间：
2004-07-01
影响因子：
3.6
通讯作者：
Wösten, HAB
中科院分区：
文献类型：
--
作者：
Claessen, D;Stokroos, I;Wösten, HAB
链霉菌形成疏水性气生菌丝，最终分隔成疏水性孢子。气生菌丝和孢子都有一个典型的表面层，称为小杆层。我们在这里提出的证据表明，小棒的形成是保守的链霉菌。小棒层的形成是罗德林和卓别林相互作用的结果。缺失rodlin基因rdlA和/或rdlB的天蓝色链霉菌菌株不再形成小棒层。相反，这些表面装饰着细纤维。删除所有8个卓别林基因（菌株DeltachpABCDEFGH）导致在气生菌丝和孢子表面的小棒层以及原纤维的情况下。除了覆盖这些表面之外，卓别林还参与菌丝逃逸到空气中，如DeltachpABCDEFGH菌株中气生菌丝数量的强烈减少所示。气生菌丝数量的减少与殖民地中rdl基因的较低表达相关。然而，每个气生菌丝的表达与野生型菌株中的表达相似，表明rdl基因的表达是在菌丝感觉到它已经生长到空气中之后开始的。
Streptomycetes form hydrophobic aerial hyphae that eventually septate into hydrophobic spores. Both aerial hyphae and spores possess a typical surface layer called the rodlet layer. We present here evidence that rodlet formation is conserved in the streptomycetes. The formation of the rodlet layer is the result of the interplay between rodlins and chaplins. A strain of Streptomyces coelicolor in which the rodlin genes rdlA and/or rdlB were deleted no longer formed the rodlet layer. Instead, these surfaces were decorated with fine fibrils. Deletion of all eight chaplin genes (strain DeltachpABCDEFGH) resulted in the absence of the rodlet layer as well as the fibrils at surfaces of aerial hyphae and spores. Apart from coating these surfaces, chaplins are involved in the escape of hyphae into the air, as was shown by the strong reduction in the number of aerial hyphae in the DeltachpABCDEFGH strain. The decrease in the number of aerial hyphae correlated with a lower expression of the rdl genes in the colony. Yet, expression per aerial hypha was similar to that in the wild-type strain, indicating that expression of the rdl genes is initiated after the hypha has sensed that it has grown into the air.