Effects of docosahexaenoic acid on some megakaryocytic cell gene expression of some enzymes controlling prostanoid synthesis
Effects of docosahexaenoic acid on some megakaryocytic cell gene expression of some enzymes controlling prostanoid synthesis
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DOI：
10.1016/j.bbrc.2008.05.155
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发表时间：
2008-08-08
影响因子：
3.1
通讯作者：
Vericel, Evelyne
中科院分区：
文献类型：
--
作者：
Guillot, Nicolas;Debard, Cyrille;Vericel, Evelyne
摄入二十二碳六烯酸(DHA)在预防心血管疾病方面的有益效果是已知的，而血小板在心血管并发症中起着关键作用。然而，高剂量的DHA可能会增加脂质过氧化并引起有害影响，尤其是在血小板中。这使得我们研究了DHA对人巨核细胞(MEG-01细胞系)中一些控制氧化还原状态和前列腺素形成的酶基因表达的影响。将MEG-01细胞与DHA(10和100mU·mol/L)共同孵育6h后，DHA使谷胱甘肽过氧化物酶-1和血栓素合成酶基因表达上调。在高浓度DHA作用下，过氧化氢酶基因表达增加，PPARβ/Delta和PPAR Gamma基因表达上调。综上所述，我们的结果支持DHA的抗氧化机制。DHA对细胞氧化还原状态的影响，与其他因素一起，可以为低剂量DHA对心血管事件的有益影响提供解释。(C)2008 Elsevier Inc.保留所有权利。
Beneficial effects of docosahexaenoic acid (DHA) intake in the prevention of cardiovascular diseases are known, and platelets play a crucial role in cardiovascular complications. However, high doses of DHA may increase lipid peroxidation and induce deleterious effects, notably in platelets. This led us to investigate the effect of DHA on gene expression of some enzymes controlling redox status and prostanoid formation in human megakaryoblastic cells (MEG-01 cell line). MEG-01 cells were incubated in presence of DHA (10 and 100 mu mol/L) for 6 h. DHA enrichment up-regulated glutathione peroxidase-1 and thromboxane synthase mRNA. DHA increased gene catalase expression and up-regulated PPAR beta/delta and PPAR gamma mRNA in presence of high concentration of DHA. In conclusion, Our results support an antioxidant mechanism of DHA. The effects of DHA on cellular redox status could, with others, provide an explanation for the beneficial influence of low consumption of DHA on cardiovascular events. (C) 2008 Elsevier Inc. All rights reserved.