Innate and adaptive immune responses to the major Parietaria allergen Par j 1 in healthy subjects
Innate and adaptive immune responses to the major Parietaria allergen Par j 1 in healthy subjects
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DOI：
10.1016/j.imbio.2012.11.006
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发表时间：
2013-07-01
期刊：
影响因子：
2.8
通讯作者：
Colombo, P.
中科院分区：
文献类型：
--
作者：
Bonura, A.;Quaratino, S.;Colombo, P.
在这项研究中，我们想分析的模式的免疫反应的Parietaria主要过敏原Par j 1新鲜纯化的外周血单核细胞（PBMC）从健康受试者。我们观察到Par j 1能够诱导健康个体中的CD 3（-）和CD 16（+）/CD 56（+）细胞产生IFN-γ。此外，多参数分析允许我们更好地定义两个IFN-γ-Par j 1特异性群体（IFN-γ（低）和IFN-γ（高）），其特征在于存在不同比例的NKT和NK细胞。我们还鉴定了IL-10（+）NK细胞亚群的伴随存在。CFSE染色显示，Par j 1蛋白可优先诱导CD 3（-）/CD 56（+）/CD 335（+）细胞增殖。最后，鉴定了CD 4（+）/CD 25（+）/FoxP 3（+）/IL-10（-）T细胞亚群。该初步研究的结果表明，在致耐受性应答期间，墙草属花粉的主要过敏原作为先天性和适应性人类免疫系统的激活剂起作用。(c)2012 Elsevier GmbH. All rights reserved.
In this study we wanted to analyse the pattern of the immune response to the Parietaria major allergen Par j 1 in freshly purified peripheral blood mononuclear cell (PBMC) from healthy subjects. We observed that Par j 1 was capable of inducing IFN-gamma production by CD3(-) and CD16(+)/CD56(+) cells exclusively in healthy individuals. Furthermore, a multiparametric analysis allowed us a better definition of two IFN-gamma-Par j 1 specific populations (IFN-gamma(dim) and IFN-gamma(high)) characterized by the presence of different proportions of NKT and NK cells. We also identified the concomitant presence of a subset of IL-10(+) NK cells. Moreover, CFSE staining showed that the Par j 1 preferentially induced the proliferation of CD3(-)/CD56(+)/CD335(+) cells. Finally, a subset of CD4(+)/CD25(+)/FoxP3(+)/IL-10(-) T cells was identified. The result of this pilot study suggest that during a tolerogenic response, the major allergen of the Parietaria pollen works as an activator of both the innate and the adaptive human immune system. (c) 2012 Elsevier GmbH. All rights reserved.