Identification of a Novel Ichthyic Parvovirus in Marine Species in Hainan Island, China
Identification of a Novel Ichthyic Parvovirus in Marine Species in Hainan Island, China
复制标题
中国海南岛海洋物种中一种新型鱼细小病毒的鉴定
DOI：
10.3389/fmicb.2019.02815
复制
发表时间：
2019-12-05
影响因子：
5.2
通讯作者：
Yin, Feifei
中科院分区：
文献类型：
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作者：
Du, Jiang;Wang, Wenqi;Yin, Feifei
细小病毒是一组多种多样的病毒，能够感染多种动物。在这项研究中，我们报告在中国海南省的鳄鱼粪便样本和罗非鱼肠道中发现了一种新的细小病毒，罗非鱼细小病毒hu - hku。利用新一代测序（NGS）技术，首次从饲养罗非鱼的鳄鱼中鉴定出这种新型细小病毒。筛选研究表明，在以罗非鱼为食的鳄鱼粪便样本中，新型细小病毒的流行率（75 ~ 86%）明显高于以鸡肉为食的鳄鱼粪便样本（4%）。进一步研究发现，海南省4个地区采集的罗非鱼粪便样本中新型细小病毒的流行率在40% ~ 90%之间。本研究通过NS1序列分析鉴定出4株新型细小病毒，4株均在罗非鱼中检出，而在鳄鱼粪便中仅检出2株。测定了罗非鱼细小病毒HMU-HKU-1的近全长基因组序列，其NS1和VP1基因与细小病毒科其他成员的氨基酸同源性分别小于45.50%和40.38%。基于完整解旋酶结构域氨基酸序列的系统发育分析表明，罗非鱼细小病毒HMU-HKU-1是根据ICTV最新的细小病毒科分类标准在Hamaparvovirinae亚科中新提出的Chaphamaparvovirus属中一个相对独立的分支。罗非鱼细小病毒HMU-HKU-1可能是Chaphamaparvovirus新属中的一个新种。罗非鱼细小病毒的鉴定进一步深入了解了细小病毒的病毒和遗传多样性，需要根据罗非鱼养殖的致病性和产量损失来评估其在罗非鱼种群中的感染情况。
Parvoviruses are a diverse group of viruses that are capable of infecting a wide range of animals. In this study, we report the discovery of a novel parvovirus, tilapia parvovirus HMU-HKU, in the fecal samples of crocodiles and intestines of tilapia in Hainan Province, China. The novel parvovirus was firstly identified from crocodiles fed with tilapia using next-generation sequencing (NGS). Screening studies revealed that the prevalence of the novel parvovirus in crocodile feces samples fed on tilapia (75–86%) was apparently higher than that in crocodiles fed with chicken (4%). Further studies revealed that the prevalence of the novel parvovirus in tilapia feces samples collected at four areas in Hainan Province was between 40 and 90%. Four stains of the novel parvovirus were identified in this study based on sequence analyses of NS1 and all the four strains were found in tilapia in contrast only two of them were detected in crocodile feces. The nearly full-length genome sequence of the tilapia parvovirus HMU-HKU-1 was determined and showed less than 45.50 and 40.38% amino acid identity with other members of Parvoviridae in NS1 and VP1 genes, respectively. Phylogenetic analysis based on the complete helicase domain amino acid sequences showed that the tilapia parvovirus HMU-HKU-1 formed a relatively independent branch in the newly proposed genus Chaphamaparvovirus in the subfamily Hamaparvovirinae according to the ICTV’s most recent taxonomic criteria for Parvoviridae classification. Tilapia parvovirus HMU-HKU-1 likely represented a new species within the new genus Chaphamaparvovirus. The identification of tilapia parvovirus HMU-HKU provides further insight into the viral and genetic diversity of parvoviruses and its infections in tilapia populations need to be evaluated in terms of pathogenicity and production losses in tilapia farming.