SPECIFICITY, CROSS-REACTIVITY AND COMPETITION PROFILE OF MONOCLONAL-ANTIBODIES TO STAPHYLOCOCCAL ENTEROTOXIN-B AND ENTEROTOXIN-C1 DETECTED BY INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAYS
SPECIFICITY, CROSS-REACTIVITY AND COMPETITION PROFILE OF MONOCLONAL-ANTIBODIES TO STAPHYLOCOCCAL ENTEROTOXIN-B AND ENTEROTOXIN-C1 DETECTED BY INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAYS
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DOI：
10.1007/bf00198011
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发表时间：
1989-01-01
影响因子：
5.4
通讯作者：
BUSCHMANN, HG
中科院分区：
文献类型：
--
作者：
KIENLE, E;BUSCHMANN, HG
本文报道了一组抗葡萄球菌肠毒素B和C1的单克隆抗体，它们与其它已知的葡萄球菌肠毒素A、C2、C3、D和E无交叉反应。在竞争ELISA中比较单克隆抗体的亲和力和表位识别。可分为三个不同的组：组1，由单克隆抗体B/3-4和B/3-8组成，以相同的方式识别葡萄球菌肠毒素B。另外，组2（MA B B/3-5）仅识别葡萄球菌肠毒素B，而组1和2在竞争ELISA中不相互竞争，因此具有不同的互补位。第3组（单克隆抗体C1/2-3和C1/4-6）只与葡萄球菌肠毒素C1反应，甚至不与葡萄球菌肠毒素C2和C3反应。
A group of monoclonal antibodies to the staphylococcal enterotoxins B and C1 without any cross-reactivity to the other known staphylococcal enterotoxins A, C2, C3, D and E was developed. The monoclonal antibodies were compared in competition ELISA''s with regard to affinity and epitope recognition. Three different groups could be classified: Group 1, consisting of the MAbs B/3-4 and B/3-8, recognizes in identical manner staphylococcal enterotoxin B. Also group 2 (MAb B/3-5) recognizes only staphylococcal enterotoxin B, yet group 1 and 2 do not compete with each other in the competition ELISA and therefore own different paratopes. Group 3 (MAb C1/2-3 and C1/4-6) reacts exclusively with staphylococcal enterotoxin C1, not even with staphylococcal enterotoxins C2 and C3.