Predictive capabilities of in vitro colon bioaccessibility for estimating in vivo relative bioavailability of arsenic from contaminated soils: Arsenic speciation and gut microbiota considerations
Predictive capabilities of in vitro colon bioaccessibility for estimating in vivo relative bioavailability of arsenic from contaminated soils: Arsenic speciation and gut microbiota considerations
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体外结肠生物可及性的预测能力，用于估计受污染土壤中砷的体内相对生物利用度：砷形态和肠道微生物群考虑因素
DOI：
10.1016/j.scitotenv.2021.151804
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发表时间：
2022
影响因子：
9.8
通讯作者：
Yanshan Cui
中科院分区：
文献类型：
--
作者：
Naiyi Yin;Xiaolin Cai;Pengfei Wang;Run Feng;Huili Du;Yaqi Fu;Guoxin Sun;Yanshan Cui
人类肠道菌群对砷（As）的转化已被证明会影响As毒性和人类健康。然而，鲜为人知的是肠道微生物群的影响，从偶然摄入的土壤作为生物利用度。在本研究中，我们采用小鼠体内模型和体外结肠期生物可利用性评价了砷的相对生物利用度（RBA）。13种砷污染土壤中砷的相对生物利用度（10.2%~ 57.7%）与结肠生物可利用度（4.8%~ 49.0%）之间存在较强的体内外相关性（R2= 0.70- 0.92，P < 0.05）。在体外培养人结肠菌群时，我们发现了高度的As转化，并且在土壤残留物中观察到65.9%的As（III）。在小鼠体内试验中，DMA（V）占尿砷累积排泄量的79.0%。除As（V）外，在小鼠粪便中还检测到As（III）、DMA（V）和As硫化物。肠道细菌（Rikenellaceae和Marinifilaceae）与小鼠砷摄入量和排泄量呈显著相关（P< 0.05）。我们的研究结果提供的证据表明，肠道微生物群可以影响转化，生物利用度，和命运的口服摄入土壤砷在人体胃肠道。
Arsenic (As) transformation by human gut microbiota has been evidenced to impact As toxicity and human health. However, little is known about the influence of gut microbiota on As bioavailability from incidental ingestion of soil. In this study, we assessed As relative bioavailability (RBA) using anin vivomouse model and As bioaccessibility in the colon phase ofin vitroassays. Strongin vivo-in vitrocorrelations (R2= 0.70–0.92,P< 0.05) were observed between soil As RBA (10.2%–57.7%) and colon bioaccessibility (4.8%–49.0%) in 13 As-contaminated soils. Uponin vitroincubation of human colon microbiota, we found a high degree of As transformation and 65.9% of generated As(III) was observed in soil residues. Forin vivomouse assay, DMA(V) accounted for 79.0% of cumulative urinary As excretion. Except for As(V), dominant As species including As(III), DMA(V) and As sulfides were also detected in mouse feces. Gut bacteria (familiesRikenellaceaeandMarinifilaceae) could be significantly correlated with As intake and excretion in mice (P< 0.05). Our findings provide evidence that gut microbiota can affect transformation, bioavailability, and fate of the orally ingested soil As in human gastrointestinal tract.