Photoelectrochemical biosensor for histone acetyltransferase detection based on ZnO quantum dots inhibited photoactivity of BiOI nanoflower
Photoelectrochemical biosensor for histone acetyltransferase detection based on ZnO quantum dots inhibited photoactivity of BiOI nanoflower
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DOI：
10.1016/j.snb.2019.127633
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发表时间：
2020-03-15
影响因子：
8.4
通讯作者：
Ai, Shiyun
中科院分区：
文献类型：
--
作者：
Chen, Yan;Zhou, Yunlei;Ai, Shiyun
组蛋白乙酰化是一种非常重要的转录后修饰，它是由乙酰辅酶A（Ac-CoA）的乙酰基供体组蛋白乙酰转移酶催化的。组蛋白乙酰化异常与多种疾病有关，因此建立一种灵敏、高效的检测组蛋白乙酰转移酶（HAT）的方法具有重要意义。本文以辅酶A乙酰化反应副产物为靶分子，建立了一种简单、灵敏的间接检测HAT的光电化学生物传感器。该传感器以BiOI纳米花为光活性材料，Fe 3 O 4-NH 2和3-马来酰亚胺丙酸为固定辅酶A的“桥”试剂，ZnO量子点为光电流抑制剂。在最佳实验条件下，随着HAT浓度在0.01 ~ 500 nM范围内变化，光电流下降。在此浓度范围内，光电流与HAT浓度的对数值呈线性关系，检出限为3 pM（S/N=3）。该传感器具有良好的检测特异性、稳定性和重现性。针对抗生素污染严重的现状，利用该光电化学生物传感器研究了抗生素对HAT活性的影响。
Histone acetylation is a very important post-transcriptional modification, which is catalyzed by histone acetyltransferase with acetyl group donor of acetyl coenzyme A (Ac-CoA). Abnormal histone acetylation is associated with many diseases, so it is important to develop a sensitive and efficient method for detecting histone acetyltransferase (HAT). In this paper, a simple and sensitive photoelectrochemical biosensor was established for indirect detection of HAT using the acetylation reaction by-product of coenzyme A (CoA) as target molecule. To construct the biosensor, BiOI nanoflower was employed as photoactive material, Fe3O4-NH2 and 3-maleimidopropionic acid were used as "bridge" reagents for immobilizing CoA, and ZnO quantum dots were adopted as photocurrent inhibitor. Under optimal experimental conditions, the photocurrent decreased with changing HAT concentration from 0.01 to 500 nM. The linear relationship between the photocurrent and the logarithm value of HAT concentration can be constructed with this concentration range and the detection limit was 3 pM (S/N=3). The sensor has excellent detection specificity, good stability and reproducibility. Based on the current situation of serious antibiotic contamination, the effect of antibiotics on HAT activity was investigated using this photoelectrochemical biosensor.