Structural Diversification of Andiconin-Derived Natural Products by α-Ketoglutarate-Dependent Dioxygenases
Structural Diversification of Andiconin-Derived Natural Products by α-Ketoglutarate-Dependent Dioxygenases
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DOI：
10.1021/acs.orglett.0c01358
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发表时间：
2020-06-05
期刊：
影响因子：
5.2
通讯作者：
Abe, Ikuro
中科院分区：
文献类型：
--
作者：
Bai, Tongxuan;Matsuda, Yudai;Abe, Ikuro
在Aspergillussp.TJ23的基因组中发现了真菌类部分萜类emeridone F（8）的生物合成基因簇，并通过表征两种α-酮戊二酸（alpha KG）依赖性双加氧酶SptF和SptN来阐明其后期生物合成。SptF催化氧化重排，然后环氧化，而SptN作为C-9羟化酶。我们的研究提供了深入了解其他andiconin（1）衍生的天然产物的生物合成机制，举例说明了α KG依赖性酶在结构复杂化中的重要作用。
The biosynthetic gene cluster of the fungal meroterpenoid emeridone F (8) was discovered in the genome of Aspergillus sp. TJ23, and its late-stage biosynthesis was elucidated by characterizing two alpha-ketoglutarate (alpha KG)-dependent dioxygenases, SptF and SptN. SptF catalyzes oxidative rearrangement followed by epoxidation, whereas SptN serves as the C-9 hydroxylase. Our study provides insight into the biosynthetic mechanisms of other andiconin (1)-derived natural products, exemplifying the important roles of alpha KG-dependent enzymes in the structural complexifications.