Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase
Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase
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DOI：
10.1038/ncb1262
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发表时间：
2005-06-01
影响因子：
21.3
通讯作者：
Gundersen, GG
中科院分区：
文献类型：
--
作者：
Ezratty, EJ;Partridge, MA;Gundersen, GG
影像学研究表明，在粘着斑解除过程中，粘着斑的微管靶向作用，尽管其分子机制尚不清楚。在这里，我们开发了一个模型系统的粘着斑拆卸的基础上发现，微管再生后诺考达唑冲洗诱导拆卸粘着斑，这种拆卸独立于Rho和Rac，但依赖于粘着斑激酶（FAK）和发动蛋白。在拆卸过程中，发动蛋白与FAK相互作用，并与局灶性粘连共定位。抑制发动蛋白可防止具有粘着斑表型的细胞迁移。我们的研究结果表明，粘着斑解体涉及微管，发动蛋白和FAK，而不是简单的逆转粘着斑形成。
Imaging studies implicate microtubule targeting of focal adhesions in focal adhesion disassembly, although the molecular mechanism is unknown. Here, we develop a model system of focal adhesion disassembly based on the finding that microtubule regrowth after nocodazole washout induces disassembly of focal adhesions, and that this disassembly occurs independently of Rho and Rac, but depends on focal adhesion kinase (FAK) and dynamin. During disassembly, dynamin interacts with FAK and colocalizes with focal adhesions. Inhibition of dynamin prevents migration of cells with a focal adhesion phenotype. Our results show that focal adhesion disassembly involves microtubules, dynamin and FAK, and is not simply the reversal of focal adhesion formation.