Magnesium incorporated chitosan based scaffolds for tissue engineering applications.
Magnesium incorporated chitosan based scaffolds for tissue engineering applications.
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DOI：
10.1016/j.bioactmat.2016.11.003
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发表时间：
2016-12
影响因子：
18.9
通讯作者：
Bhattarai N
中科院分区：
文献类型：
--
作者：
Adhikari U;Rijal NP;Khanal S;Pai D;Sankar J;Bhattarai N
壳聚糖基多孔支架具有良好的体内生物相容性、降解速度可控、力学性能可调等优点，在生物医学特别是组织工程领域具有重要的应用价值。研究了壳聚糖(CS)、羧甲基壳聚糖(CMC)和葡萄糖酸镁(MGG)生物活性支架的制备和表征。通过冷冻诱导CS、CMC和MGG的聚电解质络合物的相分离和冷冻干燥，制备了支架。支架具有均匀的孔隙率和高度互联的孔，大小范围为50-250uμm。抗压强度高达400千帕，弹性模数高达5兆帕。这些支架被发现保持完好，在体外条件下进行测试时，保留了它们原来的三维框架。这些支架对3T3成纤维细胞和成骨细胞无细胞毒性。这些观察结果证明了这种新方法在制备适合于组织工程应用的支架材料方面的有效性。成功合成了壳聚糖-镁基复合支架。三维网络分布均匀，在细胞培养介质中稳定，孔径在50-250Mμm之间。抗压强度高达400千帕，弹性模数高达5兆帕。对3T3成纤维细胞无细胞毒作用。
Chitosan based porous scaffolds are of great interest in biomedical applications especially in tissue engineering because of their excellent biocompatibility in vivo, controllable degradation rate and tailorable mechanical properties. This paper presents a study of the fabrication and characterization of bioactive scaffolds made of chitosan (CS), carboxymethyl chitosan (CMC) and magnesium gluconate (MgG). Scaffolds were fabricated by subsequent freezing-induced phase separation and lyophilization of polyelectrolyte complexes of CS, CMC and MgG. The scaffolds possess uniform porosity with highly interconnected pores of 50–250 μm size range. Compressive strengths up to 400 kPa, and elastic moduli up to 5 MPa were obtained. The scaffolds were found to remain intact, retaining their original three-dimensional frameworks while testing in in-vitro conditions. These scaffolds exhibited no cytotoxicity to 3T3 fibroblast and osteoblast cells. These observations demonstrate the efficacy of this new approach to preparing scaffold materials suitable for tissue engineering applications. Chitosan-magnesium-based composite scaffolds successfully synthesized. Uniformly distributed 3D networks, stable in cell culture medium with pore size in the range of 50–250 μm obtained. Compressive strengths up to 400 kPa and elastic moduli up to 5 MPa obtained. No cytotoxicity observed with 3T3 fibroblast cells.