Complete Genome Sequence of a Recombinant Coxsackievirus B4 from a Patient with a Fatal Case of Hand, Foot, and Mouth Disease in Guangxi, China
Complete Genome Sequence of a Recombinant Coxsackievirus B4 from a Patient with a Fatal Case of Hand, Foot, and Mouth Disease in Guangxi, China
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DOI：
10.1128/jvi.01808-12
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发表时间：
2012-10-01
影响因子：
5.4
通讯作者：
Jin, Qi
中科院分区：
文献类型：
--
作者：
Hu, Y. F.;Du, J.;Jin, Qi
柯萨奇病毒B4 （CVB4）属于小核糖核酸病毒科人类肠道病毒B种。本文报道了一株重组CVB4菌株CVB4/GX/10的全新全基因组序列，该菌株分离自中国一例手足口病致命病例。完整的基因组包括7293个核苷酸（不包括3' poly(A)尾），并有一个开放的阅读框，在核苷酸位置742和7293之间映射，编码2183个氨基酸的多蛋白。基于不同基因组区域的系统发育分析显示，CVB4/GX/10在基因组的5‘半部分（VP4类似于2B）最接近CVB4菌株EPIHFMD-CLOSE contact16，尽管它在基因组的3’半部分（2C类似于3D）更接近中国CVB5菌株CVB5/Henan/2010。此外，基于全基因组的相似bootscan分析表明，重组可能发生在2C结构域内，CVB4/GX/10可能是CVB4株EPIHFMD-CLOSE contact16和CVB5/河南/2010在共循环和进化过程中发生的型间重组的后代，这是肠道病毒中相对常见的现象。
The coxsackievirus B4 (CVB4) belongs to human enterovirus B species within the family Picornaviridae. Here we report a novel complete genome sequence of a recombinant CVB4 strain, CVB4/GX/10, which was isolated from a patient with a fatal case of hand, foot, and mouth disease in China. The complete genome consists of 7,293 nucleotides, excluding the 3' poly(A) tail, and has an open reading frame that maps between nucleotide positions 742 and 7293 and encodes a 2,183-amino-acid polyprotein. Phylogenetic analysis based on different genome regions reveals that CVB4/GX/10 is closest to a CVB4 strain, EPIHFMD-CLOSE CONTACT-16, in the 5' half (VP4 similar to 2B) of the genome, although it is closer to a Chinese CVB5 strain, CVB5/Henan/2010, in the 3' half (2C similar to 3D) of the genome. Furthermore, similar bootscan analysis based on the whole genomes demonstrates that recombination has possibly occurred within the 2C domain and that CVB4/GX/10 is a possible progeny of intertypic recombination of the CVB4 strain EPIHFMD-CLOSE CONTACT-16 and CVB5/Henan/2010 that occurred during their cocirculation and evolution, which is a relatively common phenomenon in enteroviruses.