Crystallization of the factor H-binding protein, FhbB, from the periopathogen Treponema denticola.
Crystallization of the factor H-binding protein, FhbB, from the periopathogen Treponema denticola.
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H 因子结合蛋白 FhbB 的结晶，来自周围病原体齿垢密螺旋体。
DOI：
10.1107/s1744309111011298
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Marconi,RichardT
中科院分区：
文献类型：
--
作者：
Miller,DanielP;McDowell,JohnV;Bell,JessicaK;Marconi,RichardT
齿垢密螺旋体是牙周病的主要病原体。齿垢密螺旋体通过与宿主的H因子（FH）结合来逃避补体介导的杀伤，H因子是补体旁路途径的负调节因子。齿垢密螺旋体FH结合蛋白已被鉴定并命名为因子H结合蛋白B（Fhb B）。使用柠檬酸钠和0.2M硫氰酸钠通过悬滴气相扩散法获得重组FhbB的晶体。 Fhb B晶体的衍射分辨率为1.8 μ m，空间群为P43212或P41212，晶胞参数a = B = 46.76，c = 167.68 μ m。 每个不对称单元两个FhbB分子得到的马修斯系数为2.2 3 Da−1，溶剂含量为44%。 FhbB是迄今为止发现的最小的细菌产生的FH结合蛋白。其结构的测定将提供独特的洞察FH结合所需的最小结构决定因素。
Treponema denticola is a primary etiological agent of periodontal disease. T. denticola evades complement-mediated killing by binding to the host's factor H (FH), a negative regulator of the alternative complement pathway. The T. denticola FH-binding protein has been identified and designated as factor H-binding protein B (FhbB). Crystals of recombinant FhbB were obtained by the hanging-drop vapor-diffusion method using sodium citrate and 0.2 M sodium thiocyanate. FhbB crystals diffracted to 1.8 Å resolution and belonged to space group P43212 or P41212, with unit-cell parameters a = b = 46.76, c = 167.68 Å. Two FhbB molecules per asymmetric unit gave a Matthews coefficient of 2.2 Å3 Da−1 and a solvent content of 44%. FhbB is the smallest bacterially produced FH-binding protein identified to date. Determination of its structure will provide unique insight into the minimal structural determinants required for FH binding.