STUDIES ON LYMPHOCYTE-T ACTIVATION .2. TARGET-CELLS FOR CONCANAVALIN-A-INDUCED GROWTH-FACTORS
STUDIES ON LYMPHOCYTE-T ACTIVATION .2. TARGET-CELLS FOR CONCANAVALIN-A-INDUCED GROWTH-FACTORS
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DOI：
10.1002/eji.1830090803
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
ANDERSSON, J
中科院分区：
文献类型：
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作者：
COUTINHO, A;LARSSON, EL;ANDERSSON, J
Con (concanavalin)A条件培养基（CM）中含有的生长因子维持了[小鼠]T[胸腺源]细胞的指数增长，这些细胞来源于Con A、植物血凝素、扁豆凝集素和美洲商陆有丝分裂原（PWM）刺激脾脏细胞的有丝分裂原，以及对h - 2d或I-E和i -c编码决定因子的混合淋巴细胞反应，以及非H-2 Mls位点控制反应。这些生长因子是T细胞特异性的，因为它们不刺激静息的小T淋巴细胞或B淋巴细胞，也不刺激由脂多糖或脂蛋白激活产生的B细胞。T细胞母细胞对CM的反应性似乎是细胞表面生长因子受体位点的可用性的结果，而生长因子受体位点在静息细胞中不表达，因为T细胞母细胞而不是小脾细胞吸收了CM中含有的促生长活性。诸如Con A和PWM之类的凝集素以这样一种方式干扰blast表面膜，从而抑制对生长因子的反应。Con a - cm在多种靶T细胞上的活性没有可检测到的同种异型同型限制。
Growth factors contained in Con (concanavalin)A-conditioned media (CM) maintained exponential growth in [mouse] T [thymus-derived] cell blasts derived from mitogen stimulation of spleen cells with Con A, phytohemagglutinin, lentil lectin and pokeweed mitogen (PWM), and from mixed lymphocyte reactions to H-2 D or I-E and I-C-encoded determinants and from non-H-2 Mls locus-controlled reactions. Such growth factors were T cell blast-specific, inasmuch as they did not stimulate resting, small T or B [bone marrow-derived] lymphocytes nor B cell blasts generated by lipopolysaccharide or lipoprotein activation. The responsiveness of T cell blasts to CM appears to be the result of the availability on the cell surface of an acceptor site for growth factors which is not expressed in resting cells because T cell blasts, but not small spleen cells, absorb out the growth-promoting activity contained in CM. Lectins such as Con A and PWM interfered with the blast surface membrane in such a way that they inhibited the response to growth factors. There was no detectable allotypic isotypic restriction in the activity of Con A-CM on a variety of target T cell blasts.