Adipogenic differentiation potential of rat adipose tissue-derived subpopulations of stromal cells
Adipogenic differentiation potential of rat adipose tissue-derived subpopulations of stromal cells
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DOI：
10.1016/j.bjps.2014.05.042
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发表时间：
2014-10-01
影响因子：
2.7
通讯作者：
Acil, Y.
中科院分区：
文献类型：
--
作者：
Gierloff, M.;Petersen, L.;Acil, Y.
脂肪来源的基质细胞（ASCs）大多是通过酶解、离心和贴壁生长分离出来的，导致细胞群非常异质性。因此，细胞培养中的其他细胞类型可以构成ASC群体的分化和增殖潜力。最近的研究表明，与传统的ASC分离方法相比，抗体辅助分离不同的ASC亚群具有优势。本研究的目的是探讨CD29-、CD71-、cd73 -和cd90选择的ASCs在体外的成脂分化潜力。以大鼠脂肪组织为原料，经酶解和离心得到基质血管组分（SVF）。随后，通过磁激活细胞分选（MACS）分离CD29(I)、CD71(I)、CD73(I)和CD90(I)细胞，接种到培养板中分化成成脂系。通过贴壁生长分离的ASCs仅作为对照。通过油红O染色和细胞培养上清中脂联素和瘦素浓度的定量来评估成脂分化。统计分析采用单因素方差分析（ANOVA），随后采用Scheffe事后程序。结果表明，通过MACS方法可以分离出具有不同成脂分化潜力的不同亚群。在cd29选择的ASC群体中确定了最高的成脂分化潜力，其次是未分类的ASC群体。CD71-、CD73-和cd90选择的细胞表现出最低的成脂分化潜力。综上所述，cd29选择的ASCs和未分类的ASCs具有相似的成脂分化潜力。因此，我们没有看到应用基于抗cd29的ASCs分离比使用贴壁生长的传统技术有明显的优势。然而，为了使这种干细胞来源在未来的脂肪组织工程应用中更具吸引力，对脂肪源性ASCs的分离/纯化方法的研究应该继续进行。(C) 2014年英国整形、重建和美容外科医师协会。Elsevier Ltd.出版。版权所有。
Adipose-derived stromal cells (ASCs) are mostly isolated by enzymatic digestion, centrifugation and adherent growth resulting in a very heterogeneous cell population. Therefore, other cell types in the cell culture can comprise the differentiation and proliferation potential of the ASC population. Recent studies indicated that an antibody-aided isolation of distinct ASC subpopulations provides advantages over the conventional method of ASC isolation. The aim of this study was to investigate the adipogenic differentiation potential of CD29-, CD71-, CD73-and CD90-selected ASCs in vitro.The stromal vascular fraction (SVF) was obtained from rat adipose tissue by enzymatic digestion and centrifugation. Subsequently, CD29(I), CD71(I), CD73(I) and CD90(I) cells were isolated by magnetic activated cell sorting (MACS), seeded into culture plates and differentiated into the adipogenic lineage. ASCs isolated by adherent growth only served as controls. Adipogenic differentiation was assessed by Oil Red O staining and quantification of the adiponectin and leptin concentrations in the cell culture supernatants. Statistical analysis was carried out using one-way analysis of variance (ANOVA) followed by the Scheffe's post hoc procedure.The results showed that different subpopulations with different adipogenic differentiation potentials can be isolated by the MACS procedure. The highest adipogenic differentiation potential was determined in the CD29-selected ASC population followed by the unsorted ASC population. The CD71-, CD73- and CD90-selected cells exhibited significantly the lowest adipogenic differentiation potential.In conclusion, the CD29-selected ASCs and the unsorted ASCs exhibited a similar adipogenic differentiation potential. Therefore, we do not see a clear advantage in the application of an anti-CD29-based isolation of ASCs over the conventional technique using adherent growth. However, the research on isolation/purification methods of adipogenic ASCs should continue in order to make this stem cell source even more attractive for future adipose tissue engineering applications. (C) 2014 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.