Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana:: Unique and overlapping functions of ARF7 and ARF19
Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana:: Unique and overlapping functions of ARF7 and ARF19
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DOI：
10.1105/tpc.104.028316
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发表时间：
2005-02-01
期刊：
影响因子：
11.6
通讯作者：
Theologis, A
中科院分区：
文献类型：
--
作者：
Okushima, Y;Overvoorde, PJ;Theologis, A
生长素反应因子(ARF)基因家族产物与AUXINANDOLE-3-乙酸蛋白一起调节生长素介导的转录激活/抑制。大多数ARF的生物学功能(S)还知之甚少。在这里，我们报告了23个ARF基因家族成员中的18个的T-DNA插入系的鉴定和特性。除先前发现的arf2/hss、arf3/ett、arf5/MP和arf7/nph4突变体外，大多数品系都没有表现出明显的生长表型，这表明ARF蛋白之间存在功能冗余。随后，我们产生了双突变体。ARF7 arf19具有ARF7和arf19单一突变体中没有的生长素相关表型，包括侧根形成严重受损以及下胚轴和根的向重力性异常。全局基因表达分析表明，在ARF7单突变体和ARF7 ARF19双突变体中，生长素诱导的基因表达严重受损。例如，在双重突变体中，几个基因的表达被破坏，例如编码器官横向边界结构域蛋白成员的基因和与器官大小有关的生长素调节基因。这些数据表明，ARF7和ARF19蛋白通过调节独特的和部分重叠的靶基因集在生长素介导的植物发育中发挥重要作用。这些观察结果为了解拟南芥ARF基因家族成员的独特和重叠功能提供了分子基础。
The AUXIN RESPONSE FACTOR (ARF) gene family products, together with the AUXINANDOLE-3-ACETIC ACID proteins, regulate auxin-mediated transcriptional activation/repression. The biological function(s) of most ARFs is poorly understood. Here, we report the identification and characterization of T-DNA insertion lines for 18 of the 23 ARF gene family members in Arabidopsis thaliana. Most of the lines fail to show an obvious growth phenotype except of the previously identified arf2/ hss, arf3/ett, arf5/mp, and arf7/nph4 mutants, suggesting that there are functional redundancies among the ARF proteins. Subsequently, we generated double mutants. arf7 arf19 has a strong auxin-related phenotype not observed in the arf7 and arf19 single mutants, including severely impaired lateral root formation and abnormal gravitropism in both hypocotyl and root. Global gene expression analysis revealed that auxin-induced gene expression is severely impaired in the arf7 single and arf7 arf19 double mutants. For example, the expression of several genes, such as those encoding members of LATERAL ORGAN BOUNDARIES domain proteins and AUXIN-REGULATED GENE INVOLVED IN ORGAN SIZE, are disrupted in the double mutant. The data suggest that the ARF7 and ARF19 proteins play essential roles in auxin-mediated plant development by regulating both unique and partially overlapping sets of target genes. These observations provide molecular insight into the unique and overlapping functions of ARF gene family members in Arabidopsis.