Mouse Germ Line Stem Cells Undergo Rapid and Stochastic Turnover
Mouse Germ Line Stem Cells Undergo Rapid and Stochastic Turnover
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DOI：
10.1016/j.stem.2010.05.017
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发表时间：
2010-08-06
期刊：
影响因子：
23.9
通讯作者：
Simons, Benjamin D.
中科院分区：
文献类型：
--
作者：
Klein, Allon M.;Nakagawa, Toshinori;Simons, Benjamin D.
在循环组织中，成体干细胞可能会丢失，随后被替换以确保体内平衡。为了检查干细胞替代的频率，我们分析了稳态小鼠精子发生中标记干细胞的群体动态。我们的研究结果表明，生精干细胞是不断更换，平均在2周内。该分析揭示了克隆大小分布的简单而稳健的缩放行为，表明干细胞替换是随机的，这意味着干细胞是等效的，并且同样可能丢失或繁殖以替换其邻居，而不管其克隆历史如何。干细胞替代的速度惊人的快，这一点得到了3D克隆形态学和精原细胞迁移的实时成像的实验支持。这些结果表明，短寿命的干细胞可能是哺乳动物干细胞系统的一个共同特征，并揭示了一种匹配组织中细胞增殖和损失速率的自然机制。
In cycling tissues, adult stem cells may be lost and subsequently replaced to ensure homeostasis. To examine the frequency of stem cell replacement, we analyzed the population dynamics of labeled stem cells in steady-state mouse spermatogenesis. Our results show that spermatogenic stem cells are continuously replaced, on average within 2 weeks. The analysis exposes a simple and robust scaling behavior of clone size distributions that shows stem cell replacement to be stochastic, meaning that stem cells are equipotent and equally likely to be lost or to multiply to replace their neighbors, irrespective of their clonal history. The surprisingly fast rate of stem cell replacement is supported experimentally by 3D clone morphology and by live-imaging of spermatogonial migration. These results suggest that short-lived stem cells may be a common feature of mammalian stem cell systems and reveal a natural mechanism for matching the rates of cell proliferation and loss in tissue.