Studies on Endophytic Actinomycetes ( I ) Streptomyces sp. Isolated from Rhododendron and Its Antifungal Activity
Studies on Endophytic Actinomycetes ( I ) Streptomyces sp. Isolated from Rhododendron and Its Antifungal Activity
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DOI：
10.1007/pl00012978
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发表时间：
2000-11
影响因子：
1.2
通讯作者：
M. Shimizu;Y. Nakagawa;Yukio Sato;T. Furumai;Y. Igarashi;H. Onaka;R. Yoshida;H. Kunoh
中科院分区：
文献类型：
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作者：
M. Shimizu;Y. Nakagawa;Yukio Sato;T. Furumai;Y. Igarashi;H. Onaka;R. Yoshida;H. Kunoh
为探索杜鹃花内生放线菌作为真菌病害生防剂的潜力，对杜鹃花幼株进行表面灭菌处理，作为内生放线菌的分离源。分别从根、茎和叶中分离出9株、6株和2株放线菌，根据菌落的宏观外观具有明显的特征，表明不同种类的放线菌作为共生菌或寄生菌生长在该植物的各个器官中。在琼脂培养基上，只有R-5菌株对杜鹃花的两种主要病原真菌Phytophthora cinnamomian和Pestalotiopsis sydowiana均能形成明显的抑菌圈，表明该菌株能产生抑菌物质。丙酮提取物对革兰氏阳性菌、酵母菌和丝状真菌有较宽的抗菌谱。分离株R-5经鉴定为a链霉菌。基于形态学、生理学和化学分类学特征。研究结果表明，R-5菌株是杜鹃花病害生物防治的理想菌株。
To survey endophytic actinomycetes as potential biocontrol agents against fungal diseases of rhododendron, young plants of rhododendron were surface-sterilized for use as an isolation source. Nine, six and two isolates, with distinguishing characteristics based on the macroscopic appearance of colonies, were obtained from roots, stems and leaves, respectively, suggesting that various species of actinomycetes grow in the respective organs of this plant as symbionts or parasites. On an agar medium, only isolate R-5 commonly formed a clear growth-inhibition zone against two major fungal pathogens of rhododendron,Phytophthora cinnamomiandPestalotiopsis sydowiana,indicating that this isolate can produce antifungal material(s). Acetone extracts of a liquid culture of R-5 had a broad antimicrobial spectrum against Gram-positive bacteria, yeast and filamentous fungi. Isolate R-5 was identified as aStreptomycessp. based on morphological, physiological and chemotaxonomical characteristics. The present results indicate that isolate R-5 is a suitable candidate for the biocontrol of diseases of rhododendron.