Universal non-antibody detection of protein phosphorylation using pIMAGO.
Universal non-antibody detection of protein phosphorylation using pIMAGO.
复制标题
使用 pIMAGO 进行蛋白质磷酸化的通用非抗体检测。
DOI：
10.1002/9780470559277.ch140208
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发表时间：
2015
影响因子：
--
通讯作者：
Tao,WAndy
中科院分区：
文献类型：
--
作者：
Iliuk,AntonB;Tao,WAndy
This article describes methods for a new, non‐antibody phosphorylation detection reagent, termed pIMAGO (phospho‐imaging). This novel reagent takes advantage not only of the unique properties of the soluble nanoparticles, but also of the multiple functionalities of the molecule, allowing for highly selective, sensitive, and quantitative assessment of protein phosphorylation without using radioactive isotopes or phospho‐specific antibodies. The methods allow for multiplexed detection of phosphorylation and total protein amount simultaneously. The straightforward and routine detection and quantitation of general phosphorylation on any site of any protein can be performed in western blot and ELISA formats. © 2015 by John Wiley & Sons, Inc.
