A high-throughput method for the conversion of CO2 obtained from biochemical samples to graphite in septa-sealed vials for quantification of 14C via accelerator mass spectrometry
A high-throughput method for the conversion of CO2 obtained from biochemical samples to graphite in septa-sealed vials for quantification of 14C via accelerator mass spectrometry
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DOI：
10.1021/ac026334j
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发表时间：
2003-05-01
影响因子：
7.4
通讯作者：
Murov, S
中科院分区：
文献类型：
--
作者：
Ognibene, TJ;Bench, G;Murov, S
加速器质谱作为生物科学中定量同位素比率分析的工具不断发展，这使得高通量样品制备成为必要。已经开发出一种方法，可将含碳样品中获得的二氧化碳转化为固体石墨，以便对碳 - 14进行高灵敏度和高精度的定量分析。使用带有隔垫密封的小瓶以及市售的一次性材料，消除了样品交叉污染，最大限度地减少了复杂的操作，并使每个样品的成本保持在较低水平。在常规操作中，含有0.25到10毫克总碳的样品可在约4小时内转化为石墨。一名技术人员每天工作8小时大约可制备150个样品。
The growth of accelerator mass spectrometry as a tool for quantitative isotope ratio analysis in the biosciences necessitates high-throughput sample preparation. A method has been developed to convert CO2 obtained from carbonaceous samples to solid graphite for highly sensitive and precise C-14 quantification. Septa-sealed vials are used along with commercially available disposable materials, eliminating sample cross contamination, minimizing complex handling, and keeping per sample costs low. Samples containing between 0.25 and 10 mg of total carbon can be reduced to graphite in similar to4 h in routine operation. Approximately 150 samples per 8-h day can be prepared by a single technician.