Of layers and spheres: the reaggregate approach in tissue engineering
Of layers and spheres: the reaggregate approach in tissue engineering
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DOI：
10.1016/s0166-2236(00)02036-1
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发表时间：
2002-03-01
影响因子：
15.9
通讯作者：
Willbold, E
中科院分区：
文献类型：
--
作者：
Layer, PG;Robitzki, A;Willbold, E
重新聚集的方法包括在悬浮培养中从给定组织的分散细胞中再生组织典型的三维球体。重组球被用于肿瘤、遗传、毒理学、生物杂交和神经球模型，并经常取代动物实验。一个特别有指导意义的例子是利用再聚集从禽类胚胎视网膜再生完整的层状组织。通过揭示层状组织形成的限制，这种视网膜球体可能有助于再生医学，包括基于干细胞的组织工程。
The reaggregate approach involves the regeneration of histotypical three-dimensional spheres from dispersed cells of a given tissue in suspension culture. Reaggregated spheres are used as tumour, genetic, toxicological, biohybrid and neurosphere models, and often replace animal experimentation. A particularly instructive example is the use of reaggregation to regenerate complete laminar tissue from avian embryonic retina. By revealing constraints of layered tissue formation, such retinal spheres could be instrumental for regenerative medicine, including stem cell-based tissue engineering.