New bioactive biomaterials based on quaternized chitosan
New bioactive biomaterials based on quaternized chitosan
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DOI：
10.1021/jf071717
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发表时间：
2008-03-12
影响因子：
6.1
通讯作者：
Coma, Veronique
中科院分区：
文献类型：
--
作者：
Belalia, Rachid;Grelier, Stephane;Coma, Veronique
为了提高壳聚糖的抗菌活性和理化性能，对壳聚糖进行了化学改性制备季铵盐。以N-烷基壳聚糖衍生物为原料，用碘代烷进行季铵化反应，制备了水溶性阳离子聚电解质（N，N，N-三甲基壳聚糖，TMC）。TMC通过H-1 NMR光谱进行表征;季铵化程度由H-1 NMR光谱和碘离子滴定确定。研究了羟丙基纤维素（HPC）薄膜或涂层与壳聚糖或TMC作为杀菌剂对单核细胞增生李斯特菌（Listeria monocytogenes）和鼠伤寒沙门氏菌（Salmonella typhimurium）生长的抑制作用。HPC-chitosan和HPC-TMC涂层对两种细菌的固体培养基均表现出完全抑制作用。在液体培养基中进行的实验表明，化学修饰后对无害李斯特菌的生长抑制活性提高。此外，膜的物理化学性质进行了评估，以确定其潜在的食品应用。抗菌剂的加入对HPC薄膜的防潮性能和力学性能有显著影响。
Chitosan was chemically modified to produce quaternary ammonium salts in order to improve its antimicrobial activity and physicochemical properties. Quaternization of N-alkyl chitosan derivatives was carried out using alkyl iodide to elaborate water-soluble cationic polyelectrolytes (N,N,N-trimethylchitosan, TMC). TMC was characterized by H-1 NMR spectroscopy; the quaternization degree was determined from H-1 NMR spectra and by titration of iodide ion. The antibacterial activity of hydroxypropylcellulose (HPC) films or coatings associated with chitosan or TMC as biocide was evaluated against the growth of Listeria monocytogenes and Salmonella typhimurium. The HPC-chitosan and HPC-TMC coatings exhibited a total inhibition on solid medium of both bacterial strains. Experiments conducted in liquid medium showed that the inhibitory activity against the growth of Listeria innocua was improved after chemical modification. Moreover, physicochemical properties of films were evaluated to determine their potential for food applications. The addition of the antibacterial agents showed a significant impact on the moisture barrier and mechanical properties of HPC films.