EVOLUTION OF TRIMETHYLARSINE BY A PENICILLIUM SP ISOLATED FROM AGRICULTURAL EVAPORATION POND WATER
EVOLUTION OF TRIMETHYLARSINE BY A PENICILLIUM SP ISOLATED FROM AGRICULTURAL EVAPORATION POND WATER
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DOI：
10.1016/0048-9697(91)90326-a
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发表时间：
1991-06-01
影响因子：
9.8
通讯作者：
FRANKENBERGER, WT
中科院分区：
文献类型：
--
作者：
HUYSMANS, KD;FRANKENBERGER, WT
砷在农业中用作杀虫剂和落叶剂。 在加州的中央谷，砷以天然高浓度存在于土壤中，来自海岸山脉的海洋沉积母质。 由于密集的农业灌溉，可溶性砷从土壤中沥滤出来，并积累在蒸发池中，可能对水禽和野生动物构成环境威胁。 从蒸发池水中分离出的青霉菌被发现能够甲基化并随后挥发有机砷。 本研究的主要重点是表征环境条件，包括培养基、砷底物、pH、温度以及磷酸盐、碳水化合物和氨基酸的存在对砷甲基化的影响。 采用气相色谱-火焰离子化检测器对三甲基胂进行监测，并采用气相色谱-质谱联用技术进行鉴定。 最佳三甲基胂生产的条件或添加物为：基本培养基，其中100 mg l-1甲基胂酸作为砷源，pH 5-6，孵育温度20 ℃，磷酸盐浓度0.1-50 mM（KH 2 PO 4）。 添加碳水化合物和糖酸的基本培养基抑制三甲基胂的生产。 氨基酸苯丙氨酸，异亮氨酸，谷氨酰胺促进三甲基胂生产的增强范围从10.2至11.6倍，没有氨基酸补充剂的控制。 从这项研究中获得的信息可能是有用的，在开发一种生物修复的方法，在捕获砷气体从土壤或水作为一种缓解替代方案，在砷污染的清理。
Arsenicals are used in agriculture as pesticides and defoliants. In the Central Valley of California, arsenic is present in soil at naturally high concentrations, being derived from marine sedimentary parent material of the Coastal Range. Due to intense agricultural irrigation, soluble arsenic is leached from the soil and accumulates in evaporation ponds where it may pose an environmental threat to the waterfowl and wildlife. A Penicillium sp. isolated from evaporation pond water was found to be capable of methylating and subsequently volatilizing organic arsenic. The major focus of this study was to characterize the environmental conditions, including culture media, arsenic substrates, pH, temperature, and the presence of phosphates, carbohydrates and amino acids on the methylation of arsenic. Trimethylarsine was monitored by gas chromatography (GC)-flame ionization detection and identified by GC-mass spectrometry. The conditions or additions for optimum trimethylarsine production were: a minimal medium in which 100 mg l-1 methylarsonic acid served as the arsenic source, pH 5-6, temperature of incubation 20-degrees-C, and phosphate concentration of 0.1-50 mM (KH2PO4). The addition of carbohydrates and sugar acids to the minimal medium suppressed trimethylarsine production. The amino acids phenylalanine, isoleucine, and glutamine promoted trimethylarsine production with an enhancement ranging from 10.2- to 11.6-fold over the control without amino acid supplementation. The information obtained from this study may be useful in developing a bioremediation approach in trapping the arsenic gas evolved from soil or water as a mitigation alternative in the cleanup of arsenic contamination.