Expression of tumor necrosis factor-alpha and interleukin-6 in oral squamous cell carcinoma.
Expression of tumor necrosis factor-alpha and interleukin-6 in oral squamous cell carcinoma.
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DOI：
10.1111/j.1349-7006.1999.tb00827.x
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发表时间：
1999-08
期刊：
影响因子：
--
通讯作者：
Yagihashi S
中科院分区：
文献类型：
--
作者：
Nakano Y;Kobayashi W;Sugai S;Kimura H;Yagihashi S
为探讨细胞因子在肿瘤发生发展和临床表现中的作用，我们检测了57例口腔鳞状细胞癌（OSCC）患者肿瘤组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的表达及其与病理分级和分期的关系。与正常对照组织相比，肿瘤组织的酶联免疫吸附试验表明TNF-α和IL-6蛋白浓度升高，逆转录-聚合酶链反应方法检测到mRNA水平上调。这些细胞因子及其转录物定位于基质巨噬细胞和肿瘤细胞中，特别是肿瘤组织的前部区域，可能表明肿瘤细胞主动合成这些细胞因子。较大肿瘤（T3，4）的IL-6蛋白水平显著高于小肿瘤（T1，2）（P < 0.05）。与无效组相比，在放射或抗癌药物有效预治疗的病例中，这些细胞因子的水平显著降低（TNF-α和IL-6的IIa级与IV级，P < 0.05）。因此，本研究表明，在口腔鳞癌组织中的细胞因子的表达增强。
To explore the role of cytokines in tumor development and clinical manifestations, we examined the expressions of tumor necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6) in tumor tissues obtained from 57 patients with oral squamous cell carcinoma (OSCC) and their relationships to pathological grade and staging. Enzyme‐linked immunosorbent assay on the tumor tissues demonstrated elevated concentrations of TNF‐α and IL‐6 proteins and upregulated mRNA levels were detected by the reverse transcription‐polymerase chain reaction method when compared to those in normal control tissues. These cytokines and their transcripts were localized in stromal macrophages and in the tumor cells in particular of the front area of tumor issues, possibly indicating active synthesis of these cytokines by tumor cells. Larger‐sized tumors (T3, 4) contained significantly greater levels of IL‐6 proteins than small‐sized tumors (T1, 2) (P < 0.05). The levels of these cytokines were significantly reduced in cases with effective pre‐treatment with radiation or anti‐cancer agents compared to those in the less effective group (P < 0.05, grade IIa vs. grade IV for both TNF‐α and IL‐6). The present study thus demonstrated enhanced expression of cytokines in OSCC tissues.
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DOI：
10.1084/jem.171.3.629
发表时间：
1990-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Mulé JJ;McIntosh JK;Jablons DM;Rosenberg SA
通讯作者：
Rosenberg SA
影响因子：
5.6
作者：
RAK, JW;HEGMANN, EJ;KERBEL, RS
通讯作者：
KERBEL, RS
DOI：
10.1111/j.1349-7006.1990.tb03342.x
发表时间：
1990-10-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
KITAHARA, M;KISHIMOTO, S;OKADA, M
通讯作者：
OKADA, M
TNF Alpha对乳腺癌细胞系T47D的生长停滞；细胞周期特异性和信号转导。
影响因子：
8.8
作者：
PUSZTAI, L;LEWIS, CE;MCGEE, JO
通讯作者：
MCGEE, JO
影响因子：
15.9
作者：
NAYLOR, MS;STAMP, GWH;BALKWILL, FR
通讯作者：
BALKWILL, FR