Guanine-rich telomeric sequences stimulate DNA polymerase activity in vitro.
Guanine-rich telomeric sequences stimulate DNA polymerase activity in vitro.
复制标题
富含鸟嘌呤的端粒序列可在体外刺激 DNA 聚合酶活性。
DOI：
10.1021/bi9918543
复制
发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Hardin,SH
中科院分区：
文献类型：
--
作者：
Ying,J;Bradley,RK;Jones,LB;Reddy,MS;Colbert,DT;Smalley,RE;Hardin,SH
富含鸟嘌呤的寡聚核苷酸和短的端粒DNA序列可以自结合形成G-四元组稳定的复合体。我们发现这种自结合可以发生在测序反应中，并且高阶结构刺激DNA聚合酶合成延伸的DNA链。碱基类似物被用来确定胡格斯汀碱基对是这些刺激性DNA结构中的稳定力。扫描力显微镜证实，这些寡聚体形成了四重DNA，这些延伸的四链结构可以被DNA聚合酶结合。由于鸟嘌呤四位基团稳定的结构被认为存在于体内，这种结构可能会刺激体内的DNA聚合。
Guanine-rich oligonucleotides and short telomeric DNA sequences can self-associate into G-quartet stabilized complexes. We discovered that this self-association can occur in sequencing reactions and that higher-order structures stimulate DNA polymerase to synthesize extended DNA strands. Base analogues were used to identify Hoogsteen base pairings as stabilizing forces in these stimulatory DNA structures. Scanning force microscopy confirmed that quartet-DNA was formed from these oligomers and that these extended, four-stranded structures could be bound by DNA polymerase. Since guanine quartet-stabilized structures are proposed to exist in vivo, such structures may stimulate DNA polymerization in vivo.