Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment
Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment
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DOI：
10.1007/s10815-015-0581-4
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发表时间：
2015-11-01
影响因子：
3.1
通讯作者：
Har-Vardi, I.
中科院分区：
文献类型：
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作者：
Czamanski-Cohen, J.;Sarid, O.;Har-Vardi, I.
目前的研究旨在寻找潜在的非侵入性生物标志物，这将帮助我们更多地了解在失败的辅助生殖治疗中起作用的机制。本探索性初步研究考察了体外受精（IVF）妇女血浆中无细胞DNA （CFD）与淋巴细胞端粒长度之间的关系，并将端粒长度和CFD水平与健康对照组进行了比较。在体外受精周期的三个时间点收集20名接受体外受精的妇女的血液。我们评估了CFD与端粒长度之间的关系，并控制了早晨的皮质醇水平。我们还在两个时间点（月经周期的黄体期和卵泡期）收集了10名健康对照者的血液，并比较了试管婴儿患者和健康对照者的平均端粒长度、CFD和皮质醇水平。结果显示，在几个时间点上，CFD水平和端粒长度之间存在反比关系，即使在控制皮质醇水平后，这种关系仍然显著。与健康对照组相比，接受体外受精的女性有统计学意义上更高的CFD水平和更短的端粒。端粒长度和CFD之间的关系应在更大规模的研究中进一步探讨，以揭示导致试管受精妇女端粒长度缩短和CFD升高的潜在机制。
The current research is aimed at finding potential non-invasive bio-markers that will help us learn more about the mechanisms at play in failed assisted reproduction treatment. This exploratory pilot study examined the relationship between cell-free DNA (CFD) in plasma and telomere length in lymphocytes among women undergoing in vitro fertilization (IVF) and compared telomere length and CFD levels to a healthy control group.Blood of 20 women undergoing IVF was collected at three time points during the IVF cycle. We assessed the relationship between CFD and telomere length as well as controlling for morning cortisol levels. We also collected blood of 10 healthy controls at two time points (luteal and follicular phases of the menstrual cycle) and compared mean telomere length, CFD, and cortisol levels between the IVF patients and healthy controls.The results revealed an inverse relationship between CFD levels and telomere lengths at several time points that remained significant even after controlling for cortisol levels. Women undergoing IVF had statistically significant higher levels of CFD and shorter telomeres compared to healthy controls.The relationship between telomere length and CFD should be further explored in larger studies in order to uncover potential mechanisms that cause both shortened telomere length and elevated CFD in women undergoing IVF.