Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max).
Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max).
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DOI：
10.1016/j.micres.2018.05.012
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发表时间：
2018-09
影响因子：
6.7
通讯作者：
Y. Liu;E. Wang;Y. Jiao;C. Tian;Lei Wang;Zi Jian Wang;Jianwen Guan;R. Singh;W. Chen;W. Chen
中科院分区：
文献类型：
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作者：
Y. Liu;E. Wang;Y. Jiao;C. Tian;Lei Wang;Zi Jian Wang;Jianwen Guan;R. Singh;W. Chen;W. Chen
利用定点插入质粒pVO 155对固氮慢生根瘤菌（Bradyrhizobium diazoefficiens）USDA 110的寄主范围进行了基因敲除，使其由原来的定殖根瘤寄主大豆（Glycine max）转变为混杂的不定殖根瘤寄主灌木豆科植物苦参（Sophora flavescens）。将接种到这两种豆科植物的这些突变体的共生表型与野生型菌株USDA 110感染的那些进行比较。利用pVO155质粒对14个Tn5转座子突变基因中的6个基因进行了断裂。Tn5和pVO155插入突变体均能使S.具有不同形态的低效不定结节的黄花。结果表明，在S.用pVO155插入突变体感染的黄曲霉。此外，在S. flavescens，由野生型菌株USDA 110感染。这些突变体与大豆具有共生能力，但共生效率不同程度地降低。这些发现揭示了根瘤菌与豆科植物之间复杂的相互作用，即根瘤菌与豆科植物之间的相互作用。例如，参与代谢途径、转运蛋白、趋化性和移动性的基因的突变可以改变根瘤菌的进入和类杆菌在新宿主豆科植物的根瘤内的发育。
Site-specific insertion plasmid pVO155 was used to knockout the genes involved in the alternation of host range of strainBradyrhizobium diazoefficiensUSDA 110 from its original determinate-nodule-forming host soybean (Glycine max), to promiscuous and indeterminate-nodule-forming shrubby legume sophora (Sophora flavescens). Symbiotic phenotypes of these mutants inoculated to these two legumes, were compared to those infected by wild-type strain USDA 110. Six genes of the total fourteen Tn5transposon mutated genes were broken using the pVO155 plasmid. Both Tn5and pVO155-inserted mutants could nodulateS. flavescenswith different morphologies of low-efficient indeterminate nodules. One to several rod or irregular bacteroids, containing different contents of poly-β-hydroxybutyrate or polyphosphate were found within the symbiosomes in nodulated cells ofS. flavescensinfected by the pVO155-inserted mutants. Moreover, none of bacteroids were observed in the pseudonodules ofS. flavescens, infected by wild-type strain USDA 110. These mutants had the nodulation ability with soybean but the symbiotic efficiency reduced to diverse extents. These findings enlighten the complicated interactions between rhizobia and legumes, i. e., mutation of genes involved in metabolic pathways, transporters, chemotaxis and mobility could alter the rhizobial entry and development of the bacteroid inside the nodules of a new host legume.