Increased vulnerability of auditory system to noise exposure in mdx mice
Increased vulnerability of auditory system to noise exposure in mdx mice
复制标题
DOI：
10.1097/00005537-200203000-00021
复制
发表时间：
2002-03-01
期刊：
影响因子：
2.6
通讯作者：
Hsieh, YL
中科院分区：
文献类型：
--
作者：
Chen, TJ;Chen, SS;Hsieh, YL
目的：抗肌萎缩蛋白是一种主要位于肌膜下方的细胞骨架蛋白。已知抗肌萎缩蛋白缺乏是杜氏肌营养不良症（DMD）的病因。包括大脑、视网膜和耳蜗毛细胞在内的其他组织也表达抗肌萎缩蛋白。最近，在两个家族中，与感音神经性听力损伤相关的一个基因（Xp21.2）已被定位在抗肌萎缩蛋白的定位位点内。因此，有理由认为抗肌萎缩蛋白可能在听觉功能中起作用。然而，动物研究得出了相互矛盾的结果。
研究设计：试图通过噪声暴露来阐明抗肌萎缩蛋白缺陷型mdx小鼠和对照B - 10小鼠听觉系统之间的差异。
方法：在本研究中，使用了mdx小鼠和B - 10小鼠。动物每天暴露于噪声中1个月，并通过记录脑干听觉诱发电位（BAEPs）来评估它们的听觉功能。
结果：在噪声暴露前，与B - 10小鼠相比，mdx小鼠显示出正常的BAEP阈值。噪声暴露1个月后，B - 10小鼠的听力阈值和BAEP潜伏期没有明显变化。相比之下，噪声暴露后mdx小鼠的听力阈值显著升高，BAEP峰值和峰间潜伏期延长。
结论：这些结果表明mdx小鼠更容易受到噪声损伤。这不仅涉及外周听觉系统，还涉及脑干中枢听觉通路。因此，提示抗肌萎缩蛋白在听觉系统中，特别是在噪声应激下，具有重要作用。
Objectives: Dystrophin is a cytoskeletal protein mainly found just beneath the sarcolemma. Lack of dystrophin is known to be the cause of Duchenne muscular dystrophy (DMD). Other tissues, including the brain, retina, and cochlear hair cells, also express dystrophin. Recently, a gene (Xp21.2) associated with sensorineural hearing impairment has been mapped within the localization site for dystrophin in two families. Thus, it is reasonable to assume that dystrophin may play a role in auditory function. However, animal studies have produced conflicting results. Study Design: An attempt was made to clarify the differences between the auditory systems of dystrophin-deficient mdx mice and control B-10 mice by exposure to noise. Methods: In the present study, mdx mice and B-10 mice were used. Animals were exposed daffy to noise for 1 month, and their auditory functions were evaluated by recording the brainstem auditory evoked potentials (BAEPs). Results: Before noise exposure, the mdx mouse demonstrated normal BAEP threshold when compared with the B-10 mouse. After 1 month of noise exposure, the B-10 mouse showed no apparent change in hearing threshold and BAEP latencies. In contrast, significantly increased hearing threshold and prolonged BAEP peak and interpeak latencies were observed in the mdx mouse after noise exposure. Conclusions: These results indicate that the mdr mice are more vulnerable to noise damage. This involves not only the peripheral auditory system, but also the brainstem central auditory pathway. Therefore, a significant role for dystrophin in the auditory system, especially under noise stress, is suggested.