ANALYSIS OF INORGANIC PYROPHOSPHATE AT PICOMOLE LEVEL
ANALYSIS OF INORGANIC PYROPHOSPHATE AT PICOMOLE LEVEL
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DOI：
10.1016/0003-2697(77)90510-3
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发表时间：
1977-01-01
影响因子：
2.9
通讯作者：
SUHADOLNIK, RJ
中科院分区：
文献类型：
--
作者：
CHEUNG, CP;SUHADOLNIK, RJ
A radiometric determination of PPi is described. Excess UDP[14C]glucose is used to react with PPi in a reaction catalyzed by UDP-glucose pyrophosphorylase. This reaction liberates [14C]glucose 1-phosphate, which is then further converted to 6-phosphol[14C]gluconic acid in the presence of phosphoglucose mutase and G-6-P dehydrogenase. The reaction product, 6-phosphol[14C]gluconic acid, is separated from UDP-[14C]glucose by the addition of activated charcoal, which adsorbs UDP-[14C]glucose preferentially. The sensitivity of the assay for PPi is 20 pmol.
