Formation and retention of staphylococcal biofilms on DLC and its hybrids compared to metals used as biomaterials
Formation and retention of staphylococcal biofilms on DLC and its hybrids compared to metals used as biomaterials
复制标题
DOI：
10.1016/j.colsurfb.2012.07.012
复制
发表时间：
2013-01-01
影响因子：
5.8
通讯作者：
Konttinen, Yrjo Tapio
中科院分区：
文献类型：
--
作者：
Myllymaa, Katja;Levon, Jaakko;Konttinen, Yrjo Tapio
表皮葡萄球菌和金黄色葡萄球菌引起大多数种植体相关感染，抗生素治疗往往失败，治愈需要手术干预。据推测，生物材料涂层的生物膜抗性提供了一种预防性的选择。将物理气相沉积类金刚石碳（DLC）及其聚四氟乙烯（DLC-PTFE-h）和聚二甲基硅氧烷（DLC-PDMS-h）杂化物与硅晶片上的钛（Ti）、钽（Ta）和铬（Cr）薄膜进行比较，以研究它们对由S.表皮葡萄球菌和表皮葡萄球菌。金黄色葡萄球菌。样品表面的特征在于表面形貌，接触角和zeta电位，因为这些属性可能会影响生物膜。使用calcofluor白色对生物膜进行染色，并使用形态测定法在荧光显微镜下进行分析。在预试验中选择16小时孵育;在该检查点，Ti、Ta、Cr和DLC-PDMS-h几乎完全被生物膜覆盖，但DLC和DLC-PTFE-h仅部分被S生物膜覆盖。表皮葡萄球菌（分别为88 +/-26%，p < 0.001和56 +/-39%，p < 0.001）或链球菌。金黄色葡萄球菌（81 ± 24%，p
Staphylococcus epidermidis and Staphylococcus aureus cause most of the implant-related infections.Antibiotic treatment often fails and cure requires surgical intervention. It was hypothesized that biomaterial coatings resistant to biofilms offer a preventive option. Physical vapour deposited diamond-like carbon (DLC) and its polytetrafluoroethylene (DLC-PTFE-h) and polydimethylsiloxane (DLC-PDMS-h) hybrids were compared to titanium (Ti), tantalum (Ta) and chromium (Cr) thin films on silicon wafers for their resistance against formation and/or retention of biofilms produced by S. epidermidis and S. aureus in vitro. Sample surfaces were characterized for surface topography, contact angle and zeta-potential, because such properties might affect the biofilm. Biofilm was stained using calcofluor white and analysed in fluorescence microscopy using morphometry. Sixteen hour incubation was selected in pilot tests; at this checkpoint Ti, Ta, Cr and DLC-PDMS-h were almost fully covered by biofilm, but DLC and DLC-PTFE-h were only partially biofilm coated by S. epidermidis (88 +/- 26%, p < 0.001 and 56 +/- 39%, p < 0.001, respectively) or S. aureus (81 +/- 24%, p