Expression profiles of MMP-1 and TIMP-1 in lumbar intervertebral disc degeneration
Expression profiles of MMP-1 and TIMP-1 in lumbar intervertebral disc degeneration
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DOI：
10.4238/2015.december.29.16
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Ji, J. J.
中科院分区：
文献类型：
--
作者：
Deng, B.;Ren, J. Z.;Ji, J. J.
腰椎间盘退变（IDD）是一种常见的临床病理现象，近年来已成为研究的热点。基质金属蛋白酶（MMP）是负责降解几乎所有细胞外基质蛋白（ECM）的酶。基质金属蛋白酶或组织金属蛋白酶抑制剂（TIMP）的过度表达可能会破坏ECM的动态平衡。因此，在本研究中，MMP-1和TIMP-1在腰椎IDD患者的表达水平进行了评估，试图阐明其在IDD发病机制和进展中的作用。共招募60例IDD患者作为实验组，沿着20例无椎间盘退变的腰椎损伤患者作为对照组。采集术前静脉血样本，并在手术过程中从病变处采集椎间盘组织。血清和组织中MMP-1和TIMP-1的水平分别通过酶联免疫吸附试验和免疫组化染色进行定量。IDD患者血清和组织中MMP-1水平均显著高于对照组（P < 0.05）。亚组分析显示，重度IDD患者MMP-1水平高于轻、中度IDD患者（P < 0.05）。IDD患者血清和组织中TIMP-1水平与对照组比较差异无统计学意义（P > 0.05）。这些结果表明，MMP-1的表达增加IDD，更严重的情况下，观察到更高的表达，而TIMP-1的表达在正常和退行性椎间盘中表达相似。
Lumbar intervertebral disc degeneration (IDD) is a common clinical pathology and has become a focus for research in recent years. Matrix metalloproteinases (MMPs) are enzymes responsible for the degradation of almost all extracellular matrix proteins (ECM). The over-expression of MMPs or tissue inhibitors of metalloproteinases (TIMPs) may disrupt the dynamic balance of the ECM. Therefore, in the current study, the expression levels of MMP-1 and TIMP-1 in lumbar IDD patients were evaluated in an attempt to elucidate their role in IDD pathogenesis and progression. In total, 60 IDD patients were recruited as the experimental group, along with 20 cases of lumbar vertebral injury without disc degeneration as the control group. Preoperative venous blood samples were collected, and intervertebral disc tissues were collected from the lesion during surgery. Serum and tissue levels of MMP-1 and TIMP-1 were quantified by enzyme-linked immunosorbent assay and immunohistochemical staining, respectively. Serum and tissue MMP-1 levels in IDD patients were significantly higher than those in the control group (P < 0.05). Additionally, sub-group analysis revealed that severe IDD patients had higher MMP-1 levels compared with mild or moderate IDD patients (P < 0.05). However, there were no significant differences in TIMP-1 levels in either the serum or tissues of IDD patients compared to patients in the control group (P > 0.05). These results demonstrate that MMP-1 expression is increased in IDD, with higher expression observed in more severe cases, whereas TIMP-1 expression was similarly expressed in both normal and degenerated discs.