Array of Biodegradable Microraftsfor Isolation and Implantation of Living, Adherent Cells.
Array of Biodegradable Microraftsfor Isolation and Implantation of Living, Adherent Cells.
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可生物降解的微生物阵列，用于分离和植入生物，粘附细胞。
DOI：
10.1039/c3ra41764f
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发表时间：
2013-06-28
期刊：
影响因子：
3.9
通讯作者：
Allbritton NL
中科院分区：
文献类型：
--
作者：
Wang Y;Phillips CN;Herrera GS;Sims CE;Yeh JJ;Allbritton NL
描述了一种有效分选活贴壁细胞并将其植入动物体内的新策略。使用用于微模塑聚乳酸-乙醇酸 (PLGA) 的聚二甲基硅氧烷基底制造了一系列可生物降解的微结构（微筏）。筛选各种形式的 PLGA 确定 PLGA 对于微筏制造、生物相容性和体外降解的适用性取决于分子量和乳酸/乙醇比率。铺在阵列上的细胞选择性地附着在微筏上，并且可以通过它们的荧光、形态或其他标准来识别。使用微针装置将细胞有效地从阵列中移出并收集。该平台用于从混合群体中分离特定细胞，从而建立分选目标细胞以进行直接植入的能力。作为概念证明，从阵列中分离出携带稳定表达荧光素酶的肿瘤细胞的荧光缀合微筏，并将其植入小鼠皮下。体内生物发光成像证实了受体动物体内肿瘤的生长。肿瘤组织切片的成像证明了植入的微筏的体内降解。该过程是一种分离和输送少量贴壁细胞用于动物植入的新策略，在组织修复、肿瘤诱导、干细胞体内分化和其他生物医学研究中具有潜在应用。
A new strategy for efficient sorting and implantation of viable adherent cells into animals is described. An array of biodegradable micro-structures (microrafts) was fabricated using a polydimethylsiloxane substrate for micromolding poly(lactic-co-glycolic acid) (PLGA). Screening various forms of PLGA determined that the suitability of PLGA for microraft manufacture, biocompatibility and in vitro degradation was dependent on molecular weight and lactic/glycolic ratio. Cells plated on the array selectively attached to the microrafts and could be identified by their fluorescence, morphology or other criteria. The cells were efficiently dislodged and collected from the array using a microneedle device. The platform was used to isolate specific cells from a mixed population establishing the ability to sort target cells for direct implantation. As a proof of concept, fluorescently conjugated microrafts carrying tumor cells stably expressing luciferase were isolated from an array and implanted subcutaneously into mice. In vivo bio-luminescence imaging confirmed the growth of a tumor in the recipient animals. Imaging of tissue sections from the tumors demonstrated in vivo degradation of the implanted microrafts. The process is a new strategy for isolating and delivering a small number of adherent cells for animal implantation with potential applications in tissue repair, tumor induction, in vivo differentiation of stem cells and other biomedical research.