Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules
Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules
复制标题
DOI：
10.1111/1523-1747.ep12345456
复制
发表时间：
1996-04-01
影响因子：
6.5
通讯作者：
Nuccitelli, R
中科院分区：
文献类型：
--
作者：
Sheridan, DM;Isseroff, RR;Nuccitelli, R
已经测量了在体内留下皮肤伤口的外向离子电流。这些电流产生约100 mV/mm的生理电场，其可用于引导角质形成细胞向愈合伤口迁移。我们研究了角质形成细胞迁移的基底是否调节对电迁移信号的趋电反应。将培养的人角质形成细胞接种在不同基质上：I型和IV型胶原、纤连蛋白、层粘连蛋白和组织培养塑料。通过延时视频显微镜在2小时内监测施加的直流（DC）电场对定向迁移的影响。通过计算与阳极-阴极方向相关的迁移角度的余弦来量化方向性。与对照（未施加电场）相比，在所有基质上均观察到向负极迁移，对照显示随机迁移。当场强从100 mV/mm增加时，方向响应没有显著增加然而，方向性的程度和平均净细胞移位随基底而显著变化。对于接种在I型和IV型胶原和塑料上的角质形成细胞，观察到响应于DC电场的最大阴极迁移，定向迁移反应是最少的层粘连蛋白基板上，而纤维连接蛋白上的细胞表现出的反应是中间的胶原蛋白和层粘连蛋白。这些结果表明，生理性离子电流与底层基质可能会影响皮肤伤口的愈合。
Outwardly directed ionic currents have been measured leaving skin wounds in vivo. These currents generate physiologic electric fields of approximately 100 mV/mm, which may function to direct keratinocyte migration toward the healing wound, We investigated whether the substrate on which the keratinocyte migrates modulates the galvanotactic response to an electric migratory signal, Cultured human keratinocytes were plated on different matrices: types I and IV collagen, fibronectin, laminin, and tissue culture plastic. The effect of an applied direct current (DC) electric field on directional migration was monitored by time-lapse video microscopy over a 2-h period. Directionality was quantitated by calculating the cosine of the angle of migration in relation to anodal-cathodal orientation. Migration toward the negative pole was observed on all matrices as compared with controls (no applied field), which displayed random migration. No significant increase in directional response occurred when the field strength was increased from 100 mV/mm (physiologic levels) to 400 mV/mm, The degree of directionality and the average net cell translocation, however, varied significantly with the substrate, The greatest cathodal migration in response to a DC electric field was observed with keratinocytes plated on types I and IV collagens and plastic, The directional migratory response was least on a laminin substrate, whereas cells on fibronectin demonstrated a response that was intermediate between those of collagen and laminin. These results suggest that physiologic ionic currents in concert: with the underlying matrix may influence the of skin wounds.