Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements
Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements
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DOI：
10.1016/s0166-6851(96)02816-2
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发表时间：
1997-03-01
影响因子：
1.5
通讯作者：
Tschudi, C
中科院分区：
文献类型：
--
作者：
Gunzl, A;Ullu, E;Tschudi, C
剪接的前导RNA （SL）通过提供连接到每个mRNA 5'端的SL序列，在锥虫原虫mRNA成熟过程中起着关键作用。作为更好地了解SL RNA的生物发生和功能的第一步，我们在原环型布鲁氏锥虫细胞的无细胞系统中表达了一个标记的SL RNA基因。转录在+1时开始，在添加提取物后1分钟就可以检测到。在体外以及可渗透细胞中，SL RNA基因的转录是由α -amanitin/tagetitoxin抗性复合体介导的，这表明一个介于经典RNA聚合酶II和RNA聚合酶III启动子之间的启动子。对SL RNA基因启动子结构的分析表明，调控元件位于编码区上游，与线虫的SL序列不同，布鲁氏体SL RNA基因转录不需要SL序列。(C) 1997爱思唯尔科学有限公司
The spliced leader (SL) RNA plays a key role in mRNA maturation in trypanosomatid protozoa by providing the SL sequence, which is joined to the 5' end of every mRNA. As a first step towards a better understanding of the biogenesis and function of the SL RNA, we expressed a tagged SL RNA gene in a cell-free system of procyclic Trypanosoma brucei cells. Transcription initiates at +1 and can be detected as early as 1 min after addition of extract.Transcription of the SL RNA gene in vitro, as well as in permeable cells, is mediated by an alpha-amanitin/tagetitoxin resistant complex, suggesting a promoter that is intermediate between a classical RNA polymerase II and RNA polymerase III promoter. An analysis of the promoter architecture of the SL RNA gene revealed that regulatory elements are located upstream of the coding region and that the SL sequence, in contrast to the nematode SL sequence, is not required for T. brucei SL RNA gene transcription. (C) 1997 Elsevier Science B.V.